Publication:
Molecular isolation and characterization of a novel occlusion body protein gene from Penaeus monodon nucleopolyhedrovirus

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Issued Date

2008

Resource Type

Article

File Type

application/pdf

ISSN

426822

DOI

10.1016/j.virol.2008.08.036

Other identifier(s)

2-s2.0-54549095708

Rights Holder(s)

Scopus

Bibliographic Citation

Virology. Vol 381, No.2 (2008), p.261-267

Suggested Citation

Chaivisuthangkura P., Tawilert C., Tejangkura T., Rukpratanporn S., Longyant S., Sithigorngul W., Sithigorngul P. Molecular isolation and characterization of a novel occlusion body protein gene from Penaeus monodon nucleopolyhedrovirus. Virology. Vol 381, No.2 (2008), p.261-267. doi:10.1016/j.virol.2008.08.036 Retrieved from: https://hdl.handle.net/20.500.14740/3848

Title

Molecular isolation and characterization of a novel occlusion body protein gene from Penaeus monodon nucleopolyhedrovirus

Author(s)

Chaivisuthangkura P.
 Tawilert C.
 Tejangkura T.
 Rukpratanporn S.
 Longyant S.
 Sithigorngul W.
 Sithigorngul P.

Abstract

The full-length of the occlusion body (OB) protein gene of Penaeus monodon nucleopolyhedrovirus (PemoNPV) was successfully isolated. The OB gene sequence contained an open reading frame (ORF) of 1359 nucleotides encoding a protein of 452 amino acid residues with a predicted molecular mass of 50.6 kDa. A putative late promoter element, TAAG, was identified 72 nt upstream of the translation start site. The amino acid sequences of tryptic digested peptides of PemoNPV OB protein obtained from LC-MS analysis matched quite well with various regions of deduced amino acid sequences. Recombinant PemoNPV OB proteins specifically reacted with monoclonal antibodies to PemoNPV OB protein. After comparison with nucleotide database, the PemoNPV OB ORF demonstrated 67% identity to an uncharacterized ORF of a baculovirus pathogenic for Penaeus vannamei. However, comparison against protein databases revealed no significant homology to other known proteins. To our knowledge, this PemoNPV OB gene is the first isolated and characterized gene of nucleopolyhedrovirus from shrimp. © 2008 Elsevier Inc. All rights reserved.

Subject(s)

DNA fragment
 Monoclonal antibody
 Recombinant protein
 Virus antibody
 Virus protein
 Animal tissue
 Antibody specificity
 Article
 Baculovirus
 Controlled study
 DNA library
 Gene isolation
 Nonhuman
 Nucleotide sequence
 Open reading frame
 Polyhedrosis virus
 Priority journal
 Promoter region
 Protein database
 Sequence homology
 Shrimp
 Translation initiation
 Virus gene
 Amino Acid Sequence
 Animals
 Antibodies, Viral
 Antibody Specificity
 Base Sequence
 Blotting, Western
 Chromatography, Liquid
 Gene Expression Regulation, Viral
 Gene Library
 Hepatopancreas
 Mass Spectrometry
 Molecular Sequence Data
 Nucleopolyhedrovirus
 Open Reading Frames
 Penaeidae
 Recombinant Proteins
 Sequence Alignment
 Viral Proteins
 Decapoda (Crustacea)
 Litopenaeus vannamei
 Monodon baculovirus
 Mushroom bacilliform virus
 Nucleopolyhedrovirus
 Penaeus monodon

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URI

https://hdl.handle.net/20.500.14740/3848

Collections

Scopus: Year 1983-2021

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