Publication:
Genotyping of Entamoeba nuttalli strains from the wild rhesus macaques of Myanmar and comparison with those from the wild rhesus macaques of Nepal and China

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

15671348

DOI

10.1016/j.meegid.2021.104830

Other identifier(s)

2-s2.0-85103713984

Rights Holder(s)

Scopus

Bibliographic Citation

Infection, Genetics and Evolution. Vol 92, No. (2021)

Suggested Citation

Mon H.M., Feng M., Pattanawong U., Kosuwin R., Yanagi T., Kobayashi S., Putaporntip C., Jongwutiwes S., Cheng X., Tachibana H. Genotyping of Entamoeba nuttalli strains from the wild rhesus macaques of Myanmar and comparison with those from the wild rhesus macaques of Nepal and China. Infection, Genetics and Evolution. Vol 92, No. (2021). doi:10.1016/j.meegid.2021.104830 Retrieved from: https://hdl.handle.net/20.500.14740/6015

Title

Genotyping of Entamoeba nuttalli strains from the wild rhesus macaques of Myanmar and comparison with those from the wild rhesus macaques of Nepal and China

Author(s)

Mon H.M.
 Feng M.
 Pattanawong U.
 Kosuwin R.
 Yanagi T.
 Kobayashi S.
 Putaporntip C.
 Jongwutiwes S.
 Cheng X.
 Tachibana H.

Abstract

Entamoeba nuttalli found in macaques is phylogenetically the closest species to Entamoeba histolytica and is potentially pathogenic. In this study, the prevalence of Entamoeba infections was examined in wild rhesus macaques by examining 73 and 90 fecal samples collected from two sites, Popa Taung Kalat (PTK) and Pho Win Taung (PWT), in Myanmar. The positive rates of E. nuttalli detected using PCR were 49% and 31% in PTK and PWT, respectively, but no infections of E. histolytica and E. moshkovskii were found. Entamoeba dispar was detected in 6% of samples only from PWT. Positive rates of E. chattoni and E. coli were both 70% in PWT and 67% and 79% in PTK, respectively. Six E. nuttalli strains from PTK and eight from PWT were obtained in the culture with xenic medium and then, one and two strains, respectively, were axenized and finally cloned. The genotypic analysis of serine-rich protein genes revealed two genotypes each in both sites. The genotypes found in five of six strains from PTK were similar to those from the strains found in Nepal, whereas the remaining one from PTK and two from PWT were similar to those obtained from macaques in China. The sequence of the 18S rDNA of strains with these four genotypes was identical to that of the strains from China. Six loci of tRNA-linked short tandem repeats were analyzed for further genotyping of the strains. Although there were two types in locus A-L in PTK isolates, one of each type for PTK and PWT was found in the other loci, including locus A-L in PWT strains. These results demonstrated that the E. nuttalli strains from Myanmar are closer to the strains from macaques in China rather than those from macaques in Nepal. © 2021 Elsevier B.V.

Subject(s)

DNA 18S
 Serine
 Transfer RNA
 Microsatellite DNA
 Ribosome DNA
 Transfer RNA
 Amebiasis
 Article
 China
 Comparative study
 Controlled study
 DNA sequence
 Entamoeba
 Entamoeba chattoni
 Entamoeba dispar
 Entamoeba histolytica
 Entamoeba moshkovskii
 Entamoeba nuttalli
 Escherichia coli
 Feces analysis
 Gene
 Gene locus
 Genotype
 Molecular cloning
 Myanmar
 Nepal
 Nonhuman
 Parasite identification
 Parasite isolation
 Polymerase chain reaction
 Prevalence
 Priority journal
 Rhesus monkey
 Serine rich protein gene
 Short tandem repeat
 Strain difference
 Wild animal
 Amebiasis
 Animal
 Entamoeba
 Feces
 Genetics
 Genotype
 Monkey disease
 Parasitology
 Phylogeny
 Procedures
 Animals
 China
 DNA, Ribosomal
 Entamoeba
 Entamoebiasis
 Feces
 Genotype
 Macaca mulatta
 Microsatellite Repeats
 Monkey Diseases
 Myanmar
 Nepal
 Phylogeny
 RNA, Transfer
 Sequence Analysis, DNA

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URI

https://hdl.handle.net/20.500.14740/6015

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Scopus: Year 1983-2021

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