Publication: Optimization of xylitol production by Candida tropicalis a26
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Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
1252526
Other identifier(s)
2-s2.0-85010748367
Rights Holder(s)
Scopus
Bibliographic Citation
Chiang Mai Journal of Science. Vol 44, No.1 (2017), p.50-58
Suggested Citation
Lorliam W., Akaracharanya A., Krajangsang S., Tolieng V., Tanasupawat S. Optimization of xylitol production by Candida tropicalis a26. Chiang Mai Journal of Science. Vol 44, No.1 (2017), p.50-58. Retrieved from: https://hdl.handle.net/20.500.14740/4733
Abstract
Factors affecting xylitol production by Candida tropicalis A26 was investigated using Plackett-Burman design. According to 5 variables; media ingredients (3 variables), inoculums and pH; xylose and peptone were critical parameters affected on the xylitol production. Central composite design (CCD) was applied to screen for an optimal concentration of xylose and peptone. The highest xylitol production in flask scale was 42.52 g/l when xylose and peptone were 80 g/l and 4 g/l, respectively. The statistical regression model predicted the maximum xylitol production with 43.27 g/l, while the observed one was 42.52 g/l. Moreover, the production was scale up to 5 L by using stirred fermenter under the optimized condition. The highest xylitol production and xylitol yield after 42 h cultivation were 71.59 g/l and 0.89 g/g xylose, respectively. The results of statistical model and 5 L fermenter indicated that the optimized medium increased xylitol yield up to 1.45 folds compared with an un-optimized condition. Experimental design was successful by applied for improvement of xylitol production by C. tropicalis A26. © 2017, Chiang Mai University. All rights reserved.