Publication:
An automated fast-flow/delayed paper-based platform for the simultaneous electrochemical detection of hepatitis B virus and hepatitis C virus core antigen

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

9565663

DOI

10.1016/j.bios.2021.113543

Other identifier(s)

2-s2.0-85112754391

Rights Holder(s)

Scopus

Bibliographic Citation

Biosensors and Bioelectronics. Vol 193, No. (2021)

Suggested Citation

Boonkaew S., Yakoh A., Chuaypen N., Tangkijvanich P., Rengpipat S., Siangproh W., Chailapakul O. An automated fast-flow/delayed paper-based platform for the simultaneous electrochemical detection of hepatitis B virus and hepatitis C virus core antigen. Biosensors and Bioelectronics. Vol 193, No. (2021). doi:10.1016/j.bios.2021.113543 Retrieved from: https://hdl.handle.net/20.500.14740/7944

Title

An automated fast-flow/delayed paper-based platform for the simultaneous electrochemical detection of hepatitis B virus and hepatitis C virus core antigen

Author(s)

Boonkaew S.
 Yakoh A.
 Chuaypen N.
 Tangkijvanich P.
 Rengpipat S.
 Siangproh W.
 Chailapakul O.

Abstract

Electrochemical paper-based analytical devices (ePADs) are useful analytical devices that serve as point-of-care testing (POCT) devices for various clinical biomarkers in view of their simplicity, portability, and low-cost format. However, multistep reagent manipulation usually restricts the performance of the device for end users. Herein, we developed a sequential ePAD for sequential immunosensing fluid delivery by integrating dual flow behaviors (fast-flow/delayed) within a single paper platform for the simultaneous detection of hepatitis B surface antigen (HBsAg) and hepatitis C core antigen (HCVcAg). In the present work, a fast-flow channel was used for the automated washing of unbound antigens, while a delayed channel was created to store a redox reagent for further electrochemical analysis with a single buffer loading (the analysis time can be completed within 500 s). Hence, the undesirable complex procedure of multi-step reagent manipulation is scarcely needed by the user. The detection limit of the proposed ePAD was as low as 18.2 pg mL−1 for HBsAg and 1.19 pg mL−1 for HCVcAg. In addition, this proposed ePAD was also proven to be effective in real clinical sera from patients to verify its biological applicability. The ePAD sensor shows high promise as an easy-to-use, portable, and extendable sensor for other multiplex biological assays. © 2021 Elsevier B.V.

Subject(s)

Antigens
 Channel flow
 Chemical detection
 Microfluidics
 Paper
 Viruses
 ELectrochemical detection
 Electrochemicals
 Hepatitis B surface antigen
 Hepatitis B virus
 Hepatitis C virus
 Hepatitis C virus core antigen
 Microfluidic paper-based analytical device
 Multi-step
 Paper-based analytical devices
 Point-of-care testing
 Analytic equipment
 Biological marker
 Buffer
 Graphene
 Hepatitis B core antigen
 Hepatitis B surface antigen
 Oxidoreductase
 Potassium chloride
 Hepatitis B surface antigen
 Hepatitis C antigen
 Article
 Blood sampling
 Chronoamperometry
 Cyclic voltammetry
 Electrochemical analysis
 Electrochemical detection
 Electron transport
 Hepatitis B
 Hepatitis B virus
 Hepatitis C
 Hepatitis C virus
 Human
 Impedance spectroscopy
 Microfluidics
 Point of care testing
 Genetic procedures
 Hepacivirus
 Hepatitis B
 Hepatitis B virus
 Biosensing Techniques
 Electrochemical Techniques
 Hepacivirus
 Hepatitis B
 Hepatitis B Surface Antigens
 Hepatitis B virus
 Hepatitis C Antigens
 Humans

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URI

https://hdl.handle.net/20.500.14740/7944

Collections

Scopus: Year 1983-2021

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