Publication: Structure and function of HIV-1 CRF01-AE envelope proteins from blood and genital fluid isolates
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Issued Date
2009
Resource Type
File Type
application/pdf
ISSN
1251562
Other identifier(s)
2-s2.0-67650179154
Rights Holder(s)
Scopus
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol 40, No.3 (2009), p.480-493
Suggested Citation
Horthongkham N., Athipanyasilp N., Siritantikorn S., Kantakamalakul W., Srisurapanon S., Sutthent R. Structure and function of HIV-1 CRF01-AE envelope proteins from blood and genital fluid isolates. Southeast Asian Journal of Tropical Medicine and Public Health. Vol 40, No.3 (2009), p.480-493. Retrieved from: https://hdl.handle.net/20.500.14740/3714
Abstract
The recombinant envelope protein (gpl20) of the human immunodeficiency virus type 1 (HIV-1) CRF01-AE env gene isolated from the corresponding blood (rgpl20- F36PC) and genital fluid (rgpl20-F36VC) specimens obtained from HIV infected individuals was successfully produced in both prokaryote and eukaryote cells. The yields of HIV-1 recombinant envelope proteins rgpl20-F36PC and rgpl20-F36VC produced in E. coli and in mammalian cells were 1.0 and 1.2, and 0.3 and 0.5 mg/ml, respectively. Antibody responses in mice immunized with rgpl20-F36VC protein were not significantly higher than those with rgpl20-F36PC protein. The level of antibody response in mice immunized with V3 deleted recombinant gpl20 proteins from rgpl20-F36VC and rgpl20-F36PC was not significantly different from wild type rgpl20 proteins. β- strands at the tip of the V3 loop of the HIV-1 envelope protein were predicted for the wild type genital fluid isolate but not for the wild type blood isolate. The replication capacity of both F36PC and F36VC was quite efficient. The infectivity assay of the epithelial cell line for pNL4-3/gpl20F36VC was better than for pNL4-3/gpl20F36PC. The extra β-strands in the V3 loop may be involved in cell tropism.
Subject(s)
Glycoprotein gp 120
Gp120 protein, Human immunodeficiency virus 1
Human immunodeficiency virus antibody
Recombinant protein
Animal
Article
Blood
Body fluid
Chemistry
Female
HeLa cell
Human
Human immunodeficiency virus infection
Immunology
Molecular genetics
Mouse
Vagina
Virology
Animals
Body Fluids
Female
Hela Cells
HIV Antibodies
HIV Envelope Protein gp120
HIV Infections
Humans
Mice
Molecular Sequence Data
Recombinant Proteins
Vagina
Gp120 protein, Human immunodeficiency virus 1
Human immunodeficiency virus antibody
Recombinant protein
Animal
Article
Blood
Body fluid
Chemistry
Female
HeLa cell
Human
Human immunodeficiency virus infection
Immunology
Molecular genetics
Mouse
Vagina
Virology
Animals
Body Fluids
Female
Hela Cells
HIV Antibodies
HIV Envelope Protein gp120
HIV Infections
Humans
Mice
Molecular Sequence Data
Recombinant Proteins
Vagina