Publication: Bacterial colonization and antimicrobial resistance genes in neonatal enteral feeding tubes
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Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
1686496
Other identifier(s)
2-s2.0-85064284508
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
FEMS Microbiology Ecology. Vol 95, No.4 (2019)
Suggested Citation
Taft D.H., Salinero L.K., Vongbhavit K., Kalanetra K.M., Masarweh C., Yu A., Underwood M.A., Mills D.A. Bacterial colonization and antimicrobial resistance genes in neonatal enteral feeding tubes. FEMS Microbiology Ecology. Vol 95, No.4 (2019). doi:10.1093/femsec/fiz039 Retrieved from: https://hdl.handle.net/20.500.14740/5401
Abstract
Enteral feeding is a key component of care in neonatal intensive care units (NICUs); however, feeding tubes harbor microbes. These microbes have the potential to cause disease, yet their source remains controversial and clinical recommendations to reduce feeding tube colonization are lacking. This study aims to improve our understanding of the bacteria in neonatal feeding tubes and to evaluate factors that may affect these bacteria. 16S rRNA gene sequencing was used to characterize the bacteria present in pharyngeal, esophageal, and gastric portions of feeding tubes, residual fluid of the tubes, and infant stool using samples from 47 infants. Similar distributions of taxa were observed in all samples, although beta diversity differed by sample type. Feeding tube samples had lower alpha diversity than stool samples, and alpha diversity increased with gestational age, day of life, and tube dwell time. In a subset of samples from 6 infants analyzed by whole metagenome sequencing, there was greater overlap in transferable antimicrobial resistance genes between tube and fecal samples in breast milk fed infants than in formula fed infants. These findings develop our understanding of neonatal feeding tube colonization, laying a foundation for research into methods for minimizing NICU patients' exposure to antimicrobial resistant microbes. © 2019 FEMS 2019.
Subject(s)
Antimicrobial activity
Bacterial disease
Bacterium
Colonization
Gene
Genetic analysis
Species diversity
Bacteria (microorganisms)
RNA 16S
Antibiotic resistance
Artificial milk
Bacterial genome
Bacterium
Breast milk
Classification
Devices
Enteric feeding
Feces
Genetics
Human
Isolation and purification
Microbiology
Microflora
Neonatal intensive care unit
Newborn
Bacteria
Drug Resistance, Bacterial
Enteral Nutrition
Feces
Genome, Bacterial
Humans
Infant Formula
Infant, Newborn
Intensive Care Units, Neonatal
Microbiota
Milk, Human
RNA, Ribosomal, 16S
Bacterial disease
Bacterium
Colonization
Gene
Genetic analysis
Species diversity
Bacteria (microorganisms)
RNA 16S
Antibiotic resistance
Artificial milk
Bacterial genome
Bacterium
Breast milk
Classification
Devices
Enteric feeding
Feces
Genetics
Human
Isolation and purification
Microbiology
Microflora
Neonatal intensive care unit
Newborn
Bacteria
Drug Resistance, Bacterial
Enteral Nutrition
Feces
Genome, Bacterial
Humans
Infant Formula
Infant, Newborn
Intensive Care Units, Neonatal
Microbiota
Milk, Human
RNA, Ribosomal, 16S