Publication:
Epidermal growth factor regulates the transition from basal sodium absorption to anion secretion in cultured endometrial epithelial cells

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Issued Date

2001

Resource Type

Article

File Type

application/pdf

ISSN

219541

DOI

10.1002/1097-4652(200002)186:2<243

Other identifier(s)

2-s2.0-0035152506

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Cellular Physiology. Vol 186, No.2 (2001), p.243-250

Suggested Citation

Deachapunya C., O'Grady S.M. Epidermal growth factor regulates the transition from basal sodium absorption to anion secretion in cultured endometrial epithelial cells. Journal of Cellular Physiology. Vol 186, No.2 (2001), p.243-250. doi:10.1002/1097-4652(200002)186:2<243 Retrieved from: https://hdl.handle.net/20.500.14740/6917

Title

Epidermal growth factor regulates the transition from basal sodium absorption to anion secretion in cultured endometrial epithelial cells

Author(s)

Deachapunya C.
 O'Grady S.M.

Abstract

The objective of this study was to investigate acute and long-term effects of epidermal growth factor (EGF) and transforming growth factor α (TGFα) on basal ion transport activity of glandular endometrial epithelial cells in primary culture. The effects of EGF on insulin-dependent regulation of Na+ transport across this epithelium was also investigated. Addition of 1.6 nM EGF or 2 nM TGFα to the basolateral, but not the apical, solution inhibited both basal and insulin-stimulated Na+ transport with a maximum response within 45-60 min. This effect was mimicked by the calcium ionophore ionomycin. Incubation with EGF for 4 days inhibited insulin-stimulated Na absorption in a concentration-dependent fashion with an IC50 value of 0.3 nM. Experiments using amphotericin B-permeabilized monolayers demonstrated that EGF inhibited Na transport by decreasing apical membrane Na conductance without affecting insulin-dependent stimulation of the Na+-K+ ATPase. Addition of EGF or TGFα for 24 h resulted in increased basal Cl- secretion in addition to inhibition of Na absorption. The EGF-induced increase in Cl- secretion was inhibited in part by indomethacin, suggesting that long-term regulation by EGF involves stimulation of arachidonic acid synthesis and prostaglandin release. The EGF-induced increase in indomethacin-insensitive Cl- secretion was prevented by the protein synthesis inhibitor cyclohexamide, and by the DNA transcription inhibitor actinomycin D indicating that EGF-stimulated anion secretion required DNA transcription and protein synthesis. The results of these studies demonstrated that the basal transport properties of endometrial epithelial cells are differentially regulated by EGF, TGFα, and insulin. © 2001 Wiley-Liss, Inc.

Subject(s)

Benzamil
 Chloride ion
 Cycloheximide
 Dactinomycin
 Epidermal growth factor
 Growth factor
 Indometacin
 Insulin
 Ionomycin
 Ouabain
 Sodium ion
 Transforming growth factor alpha
 Animal cell
 Article
 Controlled study
 Electrical parameters
 Endometrium
 Epithelium cell
 Ion transport
 Nonhuman
 Priority journal
 Sodium absorption
 Swine
 Uterus
 Amiloride
 Animals
 Biological Transport
 Cell Membrane
 Cell Membrane Permeability
 Cells, Cultured
 Chlorides
 Cyclooxygenase Inhibitors
 Dactinomycin
 Endometrium
 Epidermal Growth Factor
 Epithelial Cells
 Female
 Indomethacin
 Insulin
 Ionomycin
 Kinetics
 Na(+)-K(+)-Exchanging ATPase
 Nitrobenzoates
 Prostaglandins
 Sodium
 Swine
 Transforming Growth Factor alpha

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URI

https://hdl.handle.net/20.500.14740/6917

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Scopus: Year 1983-2021

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