Publication: Determination of dengue virus serotypes in Thailand using PCR based method
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Issued Date
2003
Resource Type
File Type
application/pdf
ISSN
1251562
Other identifier(s)
2-s2.0-1842684421
Rights Holder(s)
Scopus
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol 34, No.4 (2003), p.781-785
Suggested Citation
Khawsak P., Phantana S., Chansiri K. Determination of dengue virus serotypes in Thailand using PCR based method. Southeast Asian Journal of Tropical Medicine and Public Health. Vol 34, No.4 (2003), p.781-785. Retrieved from: https://hdl.handle.net/20.500.14740/6600
Author(s)
Abstract
A reverse transcriptase-polymerase chain reaction (RT-PCR) and a single-tube multiplex PCR assay was modified for typing of dengue virus in different geographical areas of Thailand during 2000-2001. A set of primers (D1 and D2) was used to generate the RT-PCR product of 511 bp in size which subsequently underwent a single-tube multiplex PCR amplification using the highly specific primers for each of the dengue virus serotypes (D1, TS1, TS2, TS3 and DEN4). The PCR products of 482, 119, 290 and 392 bp in size were generated for dengue virus serotypes 1, 2, 3, and 4, respectively. Each set of specific primers showed no amplification of non-specific and non-target PCR products from human genomic DNA. The method was applied for investigation of 637 human blood samples in Thailand during 2000-2001 and found that 71, 43, 28, and 43 patients were classified as having a single infection with serotypes 1, 2, 3, and 4, respectively. Multiple infections with two or more dengue virus serotypes were also detected.