Publication:
Improved sensitivity of Taura syndrome virus immunodetection with a monoclonal antibody against the recombinant VP2 capsid protein

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

1660934

DOI

10.1016/j.jviromet.2009.11.007

Other identifier(s)

2-s2.0-73049092057

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Virological Methods. Vol 163, No.2 (2010), p.433-439

Suggested Citation

Chaivisuthangkura P., Longyant S., Hajimasalaeh W., Sridulyakul P., Rukpratanporn S., Sithigorngul P. Improved sensitivity of Taura syndrome virus immunodetection with a monoclonal antibody against the recombinant VP2 capsid protein. Journal of Virological Methods. Vol 163, No.2 (2010), p.433-439. doi:10.1016/j.jviromet.2009.11.007 Retrieved from: https://hdl.handle.net/20.500.14740/7623

Title

Improved sensitivity of Taura syndrome virus immunodetection with a monoclonal antibody against the recombinant VP2 capsid protein

Author(s)

Chaivisuthangkura P.
 Longyant S.
 Hajimasalaeh W.
 Sridulyakul P.
 Rukpratanporn S.
 Sithigorngul P.

Abstract

Taura syndrome virus (TSV) is one of the major pathogens causing mortality in the whiteleg shrimp, Litopenaeus vannamei. In this study, the gene sequence encoding the VP2 capsid protein (40 kDa) of TSV was cloned into pMAL-C2 expression vector. Five monoclonal antibodies (MAbs) were produced against the VP2 capsid protein, which was expressed heterologously in the form of a fusion protein with maltose binding protein and called MBP-VP2. All MAbs belonged to the IgG1 subclass and could bind MBP-VP2 at 400-800 pg/spot in immuno-dot blot assays. The MAbs could detect VP2 both in extracts from shrimp infected naturally in western blotting and dot blotting and in shrimp tissues in immunohistochemistry. Additionally, these MAbs did not exhibit cross-reactivity to extracts from uninfected shrimp or shrimp infected with several other common viruses. However, the dot blot assay sensitivity for TSV was approximately 10,000 times lower than that of one step RT-PCR. The MAb TSV2-88 specific to VP2 obtained in this study demonstrated an approximately twofold higher sensitivity than that of the MAb specific to VP3 from a previous study. In immunohistochemistry, the MAb TSV2-88 specific to VP2 demonstrated stronger immunoreactivity than the MAb TSV3-601 specific to VP3. A combination of the VP2 and VP3 MAbs could be used to more easily detect TSV infections in field samples of L. vannamei with better sensitivity and fidelity than using a single MAb. © 2009 Elsevier B.V. All rights reserved.

Subject(s)

Hybrid protein
 Immunoglobulin G1
 Maltose binding protein
 Monoclonal antibody
 Protein VP2
 Protein VP3
 Animal tissue
 Antigen binding
 Article
 Cell lysate
 Controlled study
 Cross reaction
 Dot hybridization
 Gene sequence
 Immunoblotting
 Immunodetection
 Immunohistochemistry
 Immunoreactivity
 Nonhuman
 Priority journal
 Protein analysis
 Protein expression
 Real time polymerase chain reaction
 RNA virus
 RNA virus infection
 Sensitivity and specificity
 Shrimp
 Taura syndrome virus
 Virus detection
 Virus recombinant
 Western blotting
 Animals
 Antibodies, Monoclonal
 Antibodies, Viral
 Capsid Proteins
 Cloning, Molecular
 Cross Reactions
 Dicistroviridae
 Immunoblotting
 Immunohistochemistry
 Penaeidae
 Recombinant Fusion Proteins
 Reverse Transcriptase Polymerase Chain Reaction
 RNA Virus Infections
 Sensitivity and Specificity
 Sequence Analysis, DNA
 Decapoda (Crustacea)
 Litopenaeus vannamei
 Miridae
 Taura syndrome virus

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URI

https://hdl.handle.net/20.500.14740/7623

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Scopus: Year 1983-2021

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