Publication:
A polymerase chain reaction assay for the survey of bancroftian filariasis

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Issued Date

2002

Resource Type

Article

File Type

application/pdf

ISSN

1251562

Other identifier(s)

2-s2.0-0037812509

Rights Holder(s)

Scopus

Bibliographic Citation

Southeast Asian Journal of Tropical Medicine and Public Health. Vol 33, No.3 (2002), p.504-508

Suggested Citation

Chansiri K., Phantana S. A polymerase chain reaction assay for the survey of bancroftian filariasis. Southeast Asian Journal of Tropical Medicine and Public Health. Vol 33, No.3 (2002), p.504-508. Retrieved from: https://hdl.handle.net/20.500.14740/6744

Title

A polymerase chain reaction assay for the survey of bancroftian filariasis

Author(s)

Chansiri K.
 Phantana S.

Abstract

A polymerase chain reaction (PCR) assay based on a highly repeated DNA sequence found in Wuchereria bancrofti (SspI repeat) has been modified for the survey of bancroftian filariasis in expatriate workers (Myamese, Karen and Mon) from Myanmar where human filariasis is endemic. The PCR was very sensitive with the ability to detect the presence of as little as 10 pg of parasite DNA. The primers used in this PCR also showed highly specific amplification of parasite DNA without the presence of non-specific and non-target PCR products such as Brugia malayi, Plasmodium falciparum and human DNA. The primers were used to investigate filariasis in four provinces in the central and western Thailand, Samut Songkram, Ratchaburi, Nakhon Pathom and Tak during 1997-2001. Among them, Tak and Ratchaburi are the only endemic areas of bancroftian filariasis. In this field study, 1,299 human blood samples (501 from Samut Songkram, 510 from Ratchaburi, 109 from Nakhon Pathom, and 179 from Tak) were collected and screened by PCR. The result showed that 1, 2, 3, and 33 patients from Samut Songkram, Ratchaburi, Nakhon Fathom, and Tak respectively were infected with W. bancrofti. These numbers were corresponded to the prevalence rate of infection of 0.2, 0.4, 2.8, and 18.5%, respectively. The PCR was able to detect the third-stage infectious larvae (L3) from Culex quinquefasciatus, mosquito vector of the W. bancrofti, that was experimentally fed to infected patient. The PCR screening of each of field mosquito pools from two endemic areas, Ratchaburi and Tak, showed that no L3 of W. bancrofti was detected.

Subject(s)

Helminth DNA
 Primer DNA
 Adolescent
 Adult
 Animal
 Article
 Clinical trial
 Culex
 Elephantiasis
 Ethnology
 Human
 Isolation and purification
 Methodology
 Middle aged
 Migration
 Multicenter study
 Myanmar
 Parasitology
 Polymerase chain reaction
 Prevalence
 Sensitivity and specificity
 Thailand
 Wuchereria bancrofti
 Adolescent
 Adult
 Animals
 Culex
 DNA Primers
 DNA, Helminth
 Elephantiasis, Filarial
 Humans
 Middle Aged
 Myanmar
 Polymerase Chain Reaction
 Prevalence
 Sensitivity and Specificity
 Thailand
 Transients and Migrants
 Wuchereria bancrofti

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URI

https://hdl.handle.net/20.500.14740/6744

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Scopus: Year 1983-2021

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