Publication:
Predominant vascular dilatation with NOS expression in lung lower lobe of thioacetamide induced-cirrhotic rat

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Issued Date

2012

Resource Type

Article

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application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876945273

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S99-S104

Suggested Citation

Showpittapornchai U., Wattanasirichaigoon S., Pradidarcheep W. Predominant vascular dilatation with NOS expression in lung lower lobe of thioacetamide induced-cirrhotic rat. Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S99-S104. Retrieved from: https://hdl.handle.net/20.500.14740/6883

Title

Predominant vascular dilatation with NOS expression in lung lower lobe of thioacetamide induced-cirrhotic rat

Author(s)

Showpittapornchai U.
 Wattanasirichaigoon S.
 Pradidarcheep W.

Abstract

Background: About eighteen percent of cirrhotic patients come along with decreased systemic arterial oxygenation and expansion of pulmonary venous plexus which triggered by nitric oxide. The level of nitrate and iNOS significantly increase in the cirrhotic patients. However, the localization of nNOS and iNOS in the lung tissue has not yet been clarified. Objective: The present study, therefore, aimed to demonstrate the sites of expansion of pulmonary blood vessels and to localize nNOS and iNOS in the lung tissue of cirrhotic rat models induced by thioacetamide (TAA). Material and Method: The rats were divided into 5 groups. The first group was the control. The other four groups were treated with 200 mg/kg body weight of TAA 3 times per week for 1, 2, 3, or 4 month(s), respectively. At the end of each month rats in each treated group were sacrificed. Lung histology and pulmonary NOS expression was studied by light microscope and immunohistochemical technique, respectively. Results: It was found that diameter of blood vessels were highest increased in the right lower lobe of the 4-months TAA-treated group. In addition, iNOS and nNOS expression was localized at epithelium of respiratory tract, endothelium of pulmonary vessel and macrophage at this age. Conclusion: The present study demonstrated that the pulmonary blood vessels at the right lower lobe with cirrhotic background got enormous dilatation. iNOS and nNOS were immunostained at epithelium of respiratory tract, pulmonary endothelium and macrophages. Our observations suggested that enhanced NOS expression is important in the development of systemic hyperdynamic circulatory abnormalities in cirrhosis. As appearance of vasodilatation at right lower lobe of lung, it could, therefore, be evidence confirming that there was a real connection between inferior pulmonary vein and azygos vein at the embryonic period but obliterated later.

Subject(s)

Inducible nitric oxide synthase
 Neuronal nitric oxide synthase
 Nitrate
 Nitric oxide
 Thioacetamide
 Animal experiment
 Animal tissue
 Article
 Blood vessel diameter
 Controlled study
 Histology
 Immunohistochemistry
 Immunolocalization
 Liver cirrhosis
 Lung alveolus epithelium
 Lung blood vessel
 Male
 Nonhuman
 Protein expression
 Protein localization
 Rat
 Vasodilatation
 Analysis of Variance
 Animals
 Case-Control Studies
 Hepatopulmonary Syndrome
 Immunohistochemistry
 Liver Cirrhosis, Experimental
 Male
 Nitric Oxide Synthase
 Rats
 Rats, Wistar
 Thioacetamide
 Vasodilation

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https://hdl.handle.net/20.500.14740/6883

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Scopus: Year 1983-2021

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