Publication:
Humanized-monoclonal antibody against heterologous leptospira infection

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Issued Date

2009

Resource Type

Article

File Type

application/pdf

ISSN

17410126

DOI

10.1093/protein/gzp008

Other identifier(s)

2-s2.0-65349165277

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Protein Engineering, Design and Selection. Vol 22, No.5 (2009), p.305-312

Suggested Citation

Maneewatch S., Sakolvaree Y., Tapchaisri P., Saengjaruk P., Songserm T., Wongratanachewin S., Tongtawe P., Srimanote P., Chaisri U., Chaicumpa W. Humanized-monoclonal antibody against heterologous leptospira infection. Protein Engineering, Design and Selection. Vol 22, No.5 (2009), p.305-312. doi:10.1093/protein/gzp008 Retrieved from: https://hdl.handle.net/20.500.14740/6822

Title

Humanized-monoclonal antibody against heterologous leptospira infection

Author(s)

Maneewatch S.
 Sakolvaree Y.
 Tapchaisri P.
 Saengjaruk P.
 Songserm T.
 Wongratanachewin S.
 Tongtawe P.
 Srimanote P.
 Chaisri U.
 Chaicumpa W.

Abstract

Patients with leptospirosis are commonly treated with antibiotics. Jarisch-Herxheimer reaction caused by toxic bacterial substances massively released as a result of the antibiotic mediated-bacterial lysis occurs in some patients which may aggravate the existing severe clinical manifestations. In this study, a humanized-murine single-chain monoclonal antibody (HuScFv) was produced and tested as an alternative of antibiotics for treatment of leptospirosis. Complementary DNA was prepared from total RNA of a murine hybridoma clone secreting monoclonal antibody (MAb) specific to LipL32 of pathogenic Leptospira spp. The MAb had therapeutic efficacy in Leptospira challenged hamsters. The VH and VL coding sequences were amplified using the cDNA as a template. The sequences were linked to form a single-chain variable murine DNA fragment (muscFv). CDR sequences of the muscFv were grafted onto the best matching human VH and VL immunoglobulin frameworks. After cloning of the humanized murine DNA sequences (huscFv) into a phagemid vector and the vector was introduced into competent Escherichia coli, the HuScFv was produced. On the same weight basis, the HuScFv possessed equal neutralizing activities to the murine ScFv counterpart against heterologous Leptospira-mediated hemolysis in vitro and rescued hamsters from a heterologous Leptospira lethal challenge. The HuScFv antibody has high therapeutic potential as an alternative to antibiotics for human leptospirosis, especially for drug hypersensitive patients. © 2009. Published by Oxford University Press. All rights reserved.

Subject(s)

Bacterial lysis
 Clinical manifestations
 Coding sequences
 Dna fragments
 Immunotherapy
 In-vitro
 Monoclonal antibodies (mab)
 Therapeutic efficacies
 Therapeutic potentials
 Antibiotics
 Bacteriology
 Cloning
 DNA
 Escherichia coli
 Genes
 Immunology
 Monoclonal antibodies
 Nucleic acids
 RNA
 Surface plasmon resonance
 DNA sequences
 Bacterial antigen
 Complementary DNA
 Humanized murine single chain monoclonal antibody
 Immunoglobulin
 Monoclonal antibody
 Outer membrane protein LipL32
 Recombinant protein
 Unclassified drug
 Amino acid sequence
 Animal experiment
 Animal model
 Animal tissue
 Antibody engineering
 Antigen specificity
 Article
 Controlled study
 DNA sequence
 Drug synthesis
 Hamster
 Hemolysis
 Hybridoma
 In vitro study
 In vivo study
 Leptospira
 Leptospira interrogans
 Leptospirosis
 Nonhuman
 Priority journal
 Animals
 Antibodies, Monoclonal
 Bacterial Outer Membrane Proteins
 Blotting, Western
 Cloning, Molecular
 Computational Biology
 Cricetinae
 DNA Primers
 DNA, Complementary
 Electrophoresis, Polyacrylamide Gel
 Escherichia coli
 Genetic Vectors
 Humans
 Immunotherapy
 Leptospira
 Leptospirosis
 Lipoproteins
 Mice
 Neutralization Tests
 Bacteria (microorganisms)
 Cricetinae
 Escherichia coli
 Leptospira
 Murinae

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URI

https://hdl.handle.net/20.500.14740/6822

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Scopus: Year 1983-2021

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