Publication:
Partial characterization of indigo (Polygonum tinctorium Ait.) plant seeds and leaves

0
0

Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

9266690

DOI

10.1016/j.indcrop.2012.06.029

Other identifier(s)

2-s2.0-84863747425

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Industrial Crops and Products. Vol 42, No.1 (2013), p.429-439

Suggested Citation

Heo B.-G., Jang H.-G., Cho J.Y., Namiesnik J., Jastrzebski Z., Vearasilp K., González-Aguilar G., Martinez-Ayala A.L., Suhaj M., Gorinstein S. Partial characterization of indigo (Polygonum tinctorium Ait.) plant seeds and leaves. Industrial Crops and Products. Vol 42, No.1 (2013), p.429-439. doi:10.1016/j.indcrop.2012.06.029 Retrieved from: https://hdl.handle.net/20.500.14740/6704

Title

Partial characterization of indigo (Polygonum tinctorium Ait.) plant seeds and leaves

Author(s)

Heo B.-G.
 Jang H.-G.
 Cho J.Y.
 Namiesnik J.
 Jastrzebski Z.
 Vearasilp K.
 González-Aguilar G.
 Martinez-Ayala A.L.
 Suhaj M.
 Gorinstein S.

Abstract

The aim of this study was to assess the contents of indigo's bioactive compounds, its antioxidant and anticancer activities in acetone, hexane and DMSO extracts and to compare the overall bioactivity with another more used medicinal plant named prolipid. It was found that the contents of the bioactive compounds in the studied extracts from different parts of indigo plant varied (P<. 0.05): the significantly highest content of polyphenols and flavonoids was in DMSO extract of prolipid, flavanols - in acetone extract of brown seeds (P<. 0.05 in both cases) and tannins - in DMSO extract of green leaves, but not significantly (P> 0.05). Also the level of antioxidant activity was different: the highest antioxidant activity of all studied samples was in prolipid: according to ABTS, FRAP and CUPRAC tests in DMSO extract (P<. 0.05 in all 3 cases), and only in acetone extract according to DPPH was not significant (P> 0.05). The correlations between polyphenol compounds and the antioxidant activities were relatively high. DPPH kinetic measurements were used to compare and distinguish the antiradical activity among indigo extracts by multivariate analysis. The FT-IR spectroscopy evaluated the presence of polyphenols. The interaction between DMSO polyphenol extracts of indigo plant and BSA showed that indigo has a strong ability as other medicinal plants such as prolipid to quench the intrinsic fluorescence of BSA by forming complexes and was measured by 3-dimensional fluorescence (3D-FL). The highest anticancer activity was in prolipid in concentrations of 800 μg/mL against Calu-6, following by indigo brown leaves. In conclusion, organic extracts of indigo brown leaves were analyzed for their antioxidant and anticancer activities and compared with prolipid, using polyphenols composition, antioxidant activities and fluorescence properties. The indigo ability to quench the intrinsic fluorescence of BSA, relatively high content of phenolic compounds and anticancer properties can be used as medicinal plant. © 2012 Elsevier B.V.

Subject(s)

3-dimensional
 Acetone extracts
 Anticancer activities
 Anticancer properties
 Antioxidant activities
 Antiproliferative activities
 Antiradical activities
 Bioactive compounds
 Fluorescence properties
 FTIR spectroscopy
 High-content
 Indigo
 Indigo plants
 Intrinsic fluorescence
 Kinetic measurement
 Medicinal plants
 Multi variate analysis
 Organic extracts
 Partial characterization
 Phenolic compounds
 Plant seeds
 Polyphenol extract
 Polyphenols
 Prolipid
 Acetone
 Fluorescence
 Hexane
 Phenols
 Seed
 Plant extracts
 Acetone
 Alkane
 Antioxidant
 Bioactivity
 Cancer
 Comparative study
 Concentration (composition)
 Dicotyledon
 Fluorescence
 Leaf
 Medicinal plant
 Multivariate analysis
 Phenolic compound
 Pigment
 Plant extract
 Seed
 Polygonum
 Polygonum tinctorium

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6704

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By