Publication:
Cholesterol-based cationic liposome increases dsRNA protection of yellow head virus infection in Penaeus vannamei

2
0

Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

1681656

DOI

10.1016/j.jbiotec.2016.04.049

Other identifier(s)

2-s2.0-84966710072

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Biotechnology. Vol 228, (2016), p.95-102

Suggested Citation

Sanitt P., Apiratikul N., Niyomtham N., Yingyongnarongkul B.-E., Assavalapsakul W., Panyim S., Udomkit A. Cholesterol-based cationic liposome increases dsRNA protection of yellow head virus infection in Penaeus vannamei. Journal of Biotechnology. Vol 228, (2016), p.95-102. doi:10.1016/j.jbiotec.2016.04.049 Retrieved from: https://hdl.handle.net/20.500.14740/5351

Title

Cholesterol-based cationic liposome increases dsRNA protection of yellow head virus infection in Penaeus vannamei

Author(s)

Sanitt P.
 Apiratikul N.
 Niyomtham N.
 Yingyongnarongkul B.-E.
 Assavalapsakul W.
 Panyim S.
 Udomkit A.

Abstract

Protection of shrimp from yellow head virus (YHV) infection has been demonstrated by injection and oral delivery of dsRNA-YHV protease gene (dsYHV) or shrimp endogenous gene (dsRab7). However, to achieve complete viral suppression and to prolong dsRNA activity, the development of an effective dsRNA delivery system is required. In this study, four cationic liposomes were synthesized and tested for their ability to increase dsRNA efficiency. The results demonstrated that entrapping dsYHV in a cholesterol-based cationic liposome gave the best protection against YHV infection when compared with other cationic lipids. The cholesterol-based cationic liposome-dsYHV (Chol-dsYHV) complex conferred YHV protection in a dose-dependent manner. Injection with Chol-dsYHV at 0.05 μg dsYHV/g shrimp could give comparable level of YHV protection to the injection with 1.25 μg naked dsYHV/g shrimp. The shrimp injected with Chol- dsYHV at 1.25 μg dsRNA/g shrimp showed only 50% mortality at 60 days post injection whereas the naked dsYHV at the same concentration gave 90% mortality. Thus, the liposome-entrapped dsYHV could lower an effective dsRNA concentration in viral protection and prolong dsRNA activity. In addition, encapsulating dsRab7 in the cholesterol-based cationic liposome could protect the dsRab7 from enzymatic digestion, and continuous feeding the shrimp with the diet formulated with the liposome-entrapped dsRab7 for 4 days in the total of 960 μg dsRab7/g shrimp could enhance YHV protection efficiency compared with the naked dsRab7. Our studies reveal that cholesterol-based cationic liposome is a promising dsRNA carrier to enhance dsRNA efficiency in both injection and oral delivery systems. © 2016 Elsevier B.V.

Subject(s)

Cholesterol
 Complexation
 Efficiency
 Genes
 Liposomes
 RNA
 Viruses
 Cationic liposomes
 Continuous feeding
 Dose-dependent manner
 Double-stranded RNA
 Enzymatic digestions
 Protection efficiency
 RNA interference
 Shrimp
 Shellfish
 Cholesterol
 Double stranded RNA
 Liposome
 Rab7 protein
 Cholesterol
 Double stranded RNA
 Liposome
 Animal experiment
 Animal tissue
 Antiviral activity
 Article
 Controlled study
 Diet
 Dose response
 Drug synthesis
 Gene delivery system
 Genetic transfection
 Infection prevention
 Mortality
 Nanoencapsulation
 Nonhuman
 Penaeus
 Penaeus vannamei
 Priority journal
 Shrimp
 Single-stranded RNA virus
 Virus infection
 Virus inhibition
 Yellow head virus
 Yellow head virus infection
 Animal
 Chemistry
 Drug effects
 Genetics
 Metabolism
 Nidovirales
 Nidovirales Infections
 Penaeidae
 RNA interference
 Veterinary
 Virology
 Virus replication
 Animals
 Cholesterol
 Liposomes
 Nidovirales Infections
 Penaeidae
 RNA Interference
 RNA, Double-Stranded
 Roniviridae
 Virus Replication

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URI

https://hdl.handle.net/20.500.14740/5351

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Scopus: Year 1983-2021

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