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Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor

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dc.contributor.authorKampeera J.
dc.contributor.authorPasakon P.
dc.contributor.authorKaruwan C.
dc.contributor.authorArunrut N.
dc.contributor.authorSappat A.
dc.contributor.authorSirithammajak S.
dc.contributor.authorDechokiattawan N.
dc.contributor.authorSumranwanich T.
dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorOunjai P.
dc.contributor.authorChankhamhaengdecha S.
dc.contributor.authorWisitsoraat A.
dc.contributor.authorTuantranont A.
dc.contributor.authorKiatpathomchai W.
dc.date.accessioned2021-04-05T03:03:21Z
dc.date.available2021-04-05T03:03:21Z
dc.date.issued2019
dc.date.issuedBE2562
dc.description.abstractVibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens. © 2019 Elsevier B.V.
dc.format.mimetypeapplication/pdf
dc.identifier.citationBiosensors and Bioelectronics. Vol 132, (2019), p.271-278
dc.identifier.doi10.1016/j.bios.2019.02.060
dc.identifier.issn9565663
dc.identifier.other2-s2.0-85062811982
dc.identifier.urihttps://hdl.handle.net/20.500.14740/5367
dc.rights.holderมหาวิทยาลัยศรีนครินทรวิโรฒ
dc.subject.otherElectric lamps
dc.subject.otherElectrochemical electrodes
dc.subject.otherError detection
dc.subject.otherFood microbiology
dc.subject.otherGraphene
dc.subject.otherGraphite electrodes
dc.subject.otherIsotherms
dc.subject.otherMeats
dc.subject.otherRedox reactions
dc.subject.otherVoltage regulators
dc.subject.otherFood-borne pathogens
dc.subject.otherIsothermal temperature
dc.subject.otherLoop mediated isothermal amplifications
dc.subject.otherPoint of care
dc.subject.otherRedox active molecules
dc.subject.otherScreen-printed graphene electrodes
dc.subject.otherSeafood
dc.subject.otherVibrio parahaemolyticus
dc.subject.otherElectrochemical sensors
dc.subject.otherGraphene
dc.subject.otherAmplicon
dc.subject.otherArticle
dc.subject.otherBacterial gene
dc.subject.otherBacterium detection
dc.subject.otherEnvironmental temperature
dc.subject.otherLimit of detection
dc.subject.otherLoop mediated isothermal amplification
dc.subject.otherNonhuman
dc.subject.otherPoint of care testing
dc.subject.otherSea food
dc.subject.otherToxR gene
dc.subject.otherVibrio parahaemolyticus
dc.titlePoint-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85062811982&doi=10.1016%2fj.bios.2019.02.060&partnerID=40&md5=b7d558d4ad471dd724549afbb62b0306

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