Publication:
Differential effects of inorganic salts on cellulase kinetics in enzymatic saccharification of cellulose and lignocellulosic biomass

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

16157591

DOI

10.1007/s00449-021-02607-6

Other identifier(s)

2-s2.0-85115451716

Rights Holder(s)

Scopus

Bibliographic Citation

Bioprocess and Biosystems Engineering. Vol 44, No.11 (2021), p.2331-2344

Suggested Citation

Paulraj Gundupalli M., Sahithi S T A., Cheng Y.-S., Tantayotai P., Sriariyanun M. Differential effects of inorganic salts on cellulase kinetics in enzymatic saccharification of cellulose and lignocellulosic biomass. Bioprocess and Biosystems Engineering. Vol 44, No.11 (2021), p.2331-2344. doi:10.1007/s00449-021-02607-6 Retrieved from: https://hdl.handle.net/20.500.14740/8146

Title

Differential effects of inorganic salts on cellulase kinetics in enzymatic saccharification of cellulose and lignocellulosic biomass

Author(s)

Paulraj Gundupalli M.
 Sahithi S T A.
 Cheng Y.-S.
 Tantayotai P.
 Sriariyanun M.

Abstract

Inorganic salt pretreatment of lignocellulosic biomass has proven to be an efficient way to increase the efficiency of enzymatic saccharification. However, it is not clear that this improvement is the result of modification of the lignocellulosic substrate after pretreatment, or removal of inhibitor, or enhancement of cellulase or a combination of these events. Therefore, this study aimed to analyze the effects of inorganic salts on kinetics of cellulase enzymes (celluclast 1.5L and accellerase 1500). Two substrates rich in cellulose content [carboxymethylcellulose (CMC), avicel (AV)] and lignocellulose substrate [sugarcane bagasse (SB)] were considered. The enzymatic saccharification was carried with and without the addition of inorganic salts (NaCl and KCl) at 0.5 M and 1.0 M concentration. The kinetic parameters, Km and Vm, were determined to mechanically understand the pattern of inhibition and enhancement of inorganic salts on enzymatic saccharification. The kinetics parameters of celluclast 1.5L and accellerase 1500 for hydrolysis of CMC and AV with NaCl showed uncompetitive inhibition. Whereas, influences of KCl on both cellulase were differentiated to function in inhibition or enhancement modes when challenged with different substrates. On the other hand, enzymatic hydrolysis efficiencies of SB using both cellulases were enhanced under addition of NaCl and KCl, by increasing Vm of celluclast 1.5L from 0.303 to 0.635 mg/mL min (0.5 M KCl) and accellerase 1500 from 0.383 to 0.719 mg/mL min (1.0 M NaCl). The details of kinetic analysis in this work revealed the mechanism of inorganic salts on cellulase kinetics to be involved in substrate modification and removal of inhibitor. Graphic abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Subject(s)

Biomass
 Cellulose derivatives
 Efficiency
 Enzymatic hydrolysis
 Kinetics
 Lignocellulosic biomass
 Saccharification
 Sodium chloride
 Substrates
 Carboxymethylcellulose
 Different substrates
 Differential effect
 Enzymatic saccharification
 Inhibition and enhancements
 Lignocellulosic substrates
 Substrate modifications
 Uncompetitive inhibitions
 Potassium compounds
 Bagasse
 Cellulase
 Cellulose
 Inorganic compound
 Inorganic salt
 Lignin
 Lignocellulose
 Chemistry
 Enzyme specificity
 Hydrolysis
 Kinetics
 Metabolism
 Sugarcane
 Cellulase
 Cellulose
 Hydrolysis
 Inorganic Chemicals
 Kinetics
 Lignin
 Saccharum
 Salts
 Substrate Specificity

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URI

https://hdl.handle.net/20.500.14740/8146

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Scopus: Year 1983-2021

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