Publication:
Reverse-phase liquid chromatographic determination of α-lipoic acid in dietary supplements using a boron-doped diamond electrode

2
0

Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

219673

DOI

10.1016/j.chroma.2010.10.004

Other identifier(s)

2-s2.0-78249278463

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Chromatography A. Vol 1217, No.49 (2010), p.7699-7705

Suggested Citation

Siangproh W., Rattanarat P., Chailapakul O. Reverse-phase liquid chromatographic determination of α-lipoic acid in dietary supplements using a boron-doped diamond electrode. Journal of Chromatography A. Vol 1217, No.49 (2010), p.7699-7705. doi:10.1016/j.chroma.2010.10.004 Retrieved from: https://hdl.handle.net/20.500.14740/7438

Title

Reverse-phase liquid chromatographic determination of α-lipoic acid in dietary supplements using a boron-doped diamond electrode

Author(s)

Siangproh W.
 Rattanarat P.
 Chailapakul O.

Abstract

A fast liquid chromatographic separation, coupled with sensitive and straightforward detection using a boron-doped diamond (BDD) electrode, was developed and validated for the determination of α-lipoic acid in dietary supplement samples. The analysis was carried out using a reversed phase C18 (150 mm × 4.6 mm, 5 μm) column with a mobile phase consisting of a 1:1 (v/v) ratio of 0.05. M phosphate solution (pH 2.5):acetonitrile, at a flow rate of 1.0. mL/min. The detection potential obtained from hydrodynamic voltammetry was 1.05. V vs. Ag/AgCl. Under optimized conditions, the chromatographic separation was performed in less than 5. min, a good linear relationship was obtained between the current and the α-lipoic concentration within the range of 0.01-60 μg/mL (correlation coefficient of 0.9971), and a detection limit of 3.0. ng/mL was determined. Furthermore, this method was successfully applied to determine α-lipoic acid concentrations in selected commercial dietary supplement samples. The recovery of α-lipoic acid in spiked samples at 0.5, 5.0 and 30 μg/mL ranged from 94.4% to 103.6% with a relative standard deviation (RSD) of between 1.2% and 3.7%. In real samples, this developed methodology produced results that were highly correlated with the standard HPLC-UV approach. Therefore, the present method can be used for fast, selective and sensitive quantification of α-lipoic acid in dietary supplements. © 2010 Elsevier B.V.

Subject(s)

Ag/AgCl
 Boron-doped diamond electrode
 Boron-doped diamond electrodes
 Chromatographic determination
 Chromatographic separations
 Correlation coefficient
 Detection limits
 Dietary supplements
 ELectrochemical detection
 Highly-correlated
 HPLC-UV
 Hydrodynamic voltammetry
 Linear relationships
 Lipoic acids
 Liquid chromatographic separations
 Mobile phase
 Optimized conditions
 Phase liquids
 Phosphate solutions
 Real samples
 Relative standard deviations
 Reversed phase
 Spiked samples
 Acetonitrile
 Acids
 Boron
 Chemical detection
 Chromatography
 Diamonds
 Food additives
 High performance liquid chromatography
 Liquids
 Nutrition
 Vitamins
 Electrochemical electrodes
 Acetonitrile
 Boron
 Diamond
 Phosphate
 Thioctic acid
 Accuracy
 Article
 Capillary electrophoresis
 Controlled study
 Correlation coefficient
 Diet supplementation
 Electrochemical detection
 Flow rate
 Hydrodynamics
 Methodology
 Oxidation
 PH
 Potentiometry
 Priority journal
 Process optimization
 Reversed phase liquid chromatography
 Separation technique
 Validation process
 Boron
 Chromatography, High Pressure Liquid
 Chromatography, Reverse-Phase
 Diamond
 Dietary Supplements
 Electrochemical Techniques
 Electrodes
 Hydrodynamics
 Oxidation-Reduction
 Reproducibility of Results
 Sensitivity and Specificity
 Thioctic Acid

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/7438

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By