Publication:
Expression and purification of 30 kilodalton protein antigen of ARA -Burkholderia pseudomallei

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dc.contributor.authorRungpanich U.
dc.contributor.authorPongsunk S.
dc.contributor.authorEzaki T.
dc.contributor.authorEkpo P.
dc.date.accessioned2021-04-05T04:31:56Z
dc.date.available2021-04-05T04:31:56Z
dc.date.issued2009
dc.date.issuedBE2552
dc.description.abstractThe 30 kDa protein of B. pseudomallei is found in virulent Ara- but not avirulent Ara+ strain. The gene was cloned in Escherichia coli JM105 employing pInIII-C2 vector. The open reading frame was 870 nucleotides with a guanine plus cytosine content of 69.9%. Arginine was the most abundant amino acid in the protein, having a PI of 12.65. Nucleotide sequence of the gene was 96% identical to B. pseudomallei 1710b chromosome II sequence CP000125.1, encoding an oxidoreductase of the short chain dehydrogenase/ reductase family. The 30 kDa antigen was expressed as a maltose-fusion protein with a yield of 5.25 mg/l of bacterial culture.
dc.format.mimetypeapplication/pdf
dc.identifier.citationSoutheast Asian Journal of Tropical Medicine and Public Health. Vol 40, No.2 (2009), p.295-301
dc.identifier.issn1251562
dc.identifier.other2-s2.0-63149121299
dc.identifier.urihttps://hdl.handle.net/20.500.14740/3696
dc.rights.holderScopus
dc.titleExpression and purification of 30 kilodalton protein antigen of ARA -Burkholderia pseudomallei
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-63149121299&partnerID=40&md5=45207395b128cb31217ab0f33faa9bd0

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