Publication:
Electrochemical impedance-based DNA sensor using pyrrolidinyl peptide nucleic acids for tuberculosis detection

2
0

Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

32670

DOI

10.1016/j.aca.2018.07.045

Other identifier(s)

2-s2.0-85050678366

Rights Holder(s)

Scopus

Bibliographic Citation

Analytica Chimica Acta. Vol 1044, (2018), p.102-109

Suggested Citation

Teengam P., Siangproh W., Tuantranont A., Vilaivan T., Chailapakul O., Henry C.S. Electrochemical impedance-based DNA sensor using pyrrolidinyl peptide nucleic acids for tuberculosis detection. Analytica Chimica Acta. Vol 1044, (2018), p.102-109. doi:10.1016/j.aca.2018.07.045 Retrieved from: https://hdl.handle.net/20.500.14740/5740

Title

Electrochemical impedance-based DNA sensor using pyrrolidinyl peptide nucleic acids for tuberculosis detection

Author(s)

Teengam P.
 Siangproh W.
 Tuantranont A.
 Vilaivan T.
 Chailapakul O.
 Henry C.S.

Abstract

A label-free electrochemical DNA sensor based on pyrrolidinyl peptide nucleic acid (acpcPNA)-immobilized on a paper-based analytical device (PAD) was developed. Unlike previous PNA-based electrochemical PAD (ePAD) sensors where the capture element was placed directly on the electrode, acpcPNA was covalently immobilized onto partially oxidized cellulose paper allowing regeneration by simple PAD replacement. As an example application, a sensor probe was designed for Mycobacterium tuberculosis (MTB) detection. The ePAD DNA sensor was used to determine a synthetic 15-base oligonucleotide of MTB by measuring the fractional change in the charge transfer resistance (R ct ) obtained from electrochemical impedance spectroscopy (EIS). The R ct of [Fe(CN) 6 ] 3-/4- before and after hybridization with the target DNA could be clearly distinguished. Cyclic voltammetry (CV) was used to verify the EIS results, and showed an increase in peak potential splitting in a similar stepwise manner for each immobilization step. Under optimal conditions, a linear calibration curve in the range of 2–200 nM and the limit of detection 1.24 nM were measured. The acpcPNA probe exhibited very high selectivity for complementary oligonucleotides over single-base-mismatch, two-base-mismatch and non-complementary DNA targets due to the conformationally constrained structure of the acpcPNA. Moreover, the ePAD DNA sensor platform was successfully applied to detect PCR-amplified MTB DNA extracted from clinical samples. The proposed paper-based electrochemical DNA sensor has potential to be an alternative device for low-cost, simple, label-free, sensitive and selective DNA sensor. © 2018 Elsevier B.V.

Subject(s)

Analytic equipment
 Charge transfer
 Cyclic voltammetry
 DNA
 Electrochemical impedance spectroscopy
 Oligonucleotides
 Paper
 Peptides
 Polymerase chain reaction
 Probes
 Spectroscopy
 AcpcPNA
 Charge transfer resistance
 Electrochemical DNA biosensors
 Electrochemical DNA sensor
 Electrochemical impedance
 Linear calibration curve
 Mycobacterium tuberculosis
 Paper-based analytical devices
 Nucleic acids
 Complementary DNA
 DNA hybrid
 Oligonucleotide
 Peptide nucleic acid
 Peptide nucleic acid
 Pyrrolidine derivative
 Article
 Base mispairing
 Cyclic potentiometry
 DNA determination
 DNA hybridization
 DNA probe
 DNA structure
 Human
 Impedance spectroscopy
 Limit of detection
 Mycobacterium tuberculosis
 Nonhuman
 Nucleic acid immobilization
 Polymerase chain reaction
 Priority journal
 RNA analysis
 Tuberculosis
 Chemistry
 Electrochemical analysis
 Genetic procedures
 Isolation and purification
 Charge Transfer
 Measuring Instruments
 Nucleic Acids
 Paper
 Peptides
 Spectroscopy
 Biosensing Techniques
 Electrochemical Techniques
 Mycobacterium tuberculosis
 Peptide Nucleic Acids
 Pyrrolidines

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URI

https://hdl.handle.net/20.500.14740/5740

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Scopus: Year 1983-2021

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