Publication: Processing and characterization of antibacterial hydrogel sheet dressings composed of poly(vinyl alcohol) and silk fibroin for wound healing application
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Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
16863933
Other identifier(s)
2-s2.0-85067098557
Rights Holder(s)
Scopus
Bibliographic Citation
Walailak Journal of Science and Technology. Vol 16, No.5 (2019), p.349-359
Suggested Citation
Uttayarat P., Chiangnoon R., Eamsiri J., Senawongse W. Processing and characterization of antibacterial hydrogel sheet dressings composed of poly(vinyl alcohol) and silk fibroin for wound healing application. Walailak Journal of Science and Technology. Vol 16, No.5 (2019), p.349-359. Retrieved from: https://hdl.handle.net/20.500.14740/5364
Author(s)
Abstract
Hydrogels are soft materials that contain high water content within their 3-dimensional structure. Such extremely hydrated environment allows hydrogels to recapitulate the structure of many native tissues inside the body. In biomedical application, hydrogels have been extensively used as biocompatible materials, drug delivery systems, and tissue-engineered scaffolds that can be designed to possess either permanent or slow-degradation properties. In this research, we applied gamma irradiation to develop transparent and conformal hydrogel sheets with sufficient mechanical strength from poly(vinyl alcohol) (PVA) and further modified the based PVA matrix with naturally-derived silk fibroin (SF) protein and silver nitrate (AgNO3) for wound healing purpose. The physical and mechanical properties of based PVA hydrogels formed at varied irradiation doses from 10 - 80 kGy were first characterized. The dose of 60 kGy was found to be optimal to process flexible and elastic PVA sheets with equilibrium degree of swelling of 1000 %, gel fraction of 90 %, and tensile strength of 19 kPa. To further enhance water absorption capacity, 10 - 40 % (w/w) silk fibroin was added to the based PVA matrix. Based on water absorption and gel fraction data, hydrogel sheets with 8PVA: 2SF formulation was selected for antibacterial test. Disc diffusion assay showed that the incorporation of 0.4 mM AgNO3 in 8PVA: 2SF hydrogel sheets could inhibit the growth of Staphyllococcus aureus and Pseudomonas aeruginosa. These results demonstrated that a prototypic, antibacterial hydrogel sheet dressing composed of both synthetic and natural polymers could be developed within a single-step by gamma irradiation technique. © 2019, Walailak University. All rights reserved.