Publication: Production of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application
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Issued Date
1996
Resource Type
File Type
application/pdf
ISSN
0125877X
Other identifier(s)
2-s2.0-0030418721
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol 14, No.1 (1996), p.43-47
Suggested Citation
Pongsunk S., Ekpo P., Dharakul T. Production of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application. Asian Pacific Journal of Allergy and Immunology. Vol 14, No.1 (1996), p.43-47. Retrieved from: https://hdl.handle.net/20.500.14740/7386
Author(s)
Abstract
Hybridomas secreting monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei were produced by immunizing BALB/cJ mice with crude culture filtrate of B. pseudomallei. Two monoclonal antibodies were found to be highly specific to B. pseudomallei as tested by indirect enzyme- linked immunosorbent assay and immunoblotting against a panel of crude whole cell extracts from B. pseudomallei, B. cepacia, Pseudomonas aeruginosa, P. putida, and Escherichia coil. One of the specific MAbs, clone SP- M, IgM subclass, could directly agglutinate all 42 B. pseudomallei, isolates. The study has shown that the agglutinating MAb has potential for rapid identification of B. pseudomallei in primary bacterial culture from clinical specimens. The antibody can be used in bacteriology laboratories to reduce time of biochemical methods, which require a few days.
Subject(s)
Bacterium antibody
Monoclonal antibody
Article
Bacterium identification
Burkholderia pseudomallei
Enzyme linked immunosorbent assay
Escherichia coli
Hybridoma
Immunoblotting
Nonhuman
Pseudomonas aeruginosa
Pseudomonas putida
Agglutination
Animals
Antibodies, Monoclonal
Burkholderia pseudomallei
Enzyme-Linked Immunosorbent Assay
Mice
Rabbits
Monoclonal antibody
Article
Bacterium identification
Burkholderia pseudomallei
Enzyme linked immunosorbent assay
Escherichia coli
Hybridoma
Immunoblotting
Nonhuman
Pseudomonas aeruginosa
Pseudomonas putida
Agglutination
Animals
Antibodies, Monoclonal
Burkholderia pseudomallei
Enzyme-Linked Immunosorbent Assay
Mice
Rabbits