Publication: Cytotoxic alkaloids against human colon adenocarcinoma cell line (HT-29) from the seed embryos of Nelumbo nucifera
2
0
Issued Date
2018
Resource Type
File Type
application/pdf
ISSN
10542523
Other identifier(s)
2-s2.0-85034226641
Rights Holder(s)
Scopus
Bibliographic Citation
Medicinal Chemistry Research. Vol 27, No.3 (2018), p.939-943
Suggested Citation
Chaichompoo W., Chokchaisiri R., Apiratikul N., Chairoungdua A., Yingyongnarongkul B.-E., Chunglok W., Tocharus C., Suksamrarn A. Cytotoxic alkaloids against human colon adenocarcinoma cell line (HT-29) from the seed embryos of Nelumbo nucifera. Medicinal Chemistry Research. Vol 27, No.3 (2018), p.939-943. doi:10.1007/s00044-017-2115-3 Retrieved from: https://hdl.handle.net/20.500.14740/3688
Abstract
Phytochemical investigation of the CHCl3 extract of the seed embryos of Nelumbo nucifera Gaertn resulted in the isolation of a new naturally occurring bisbenzylisoquinoline alkaloid, O-methylneferine (1), together with five known alkaloids, neferine (2), armepavine (3), (–)-(1R)-N-methylcoclaurine (4), nuciferine (5), and pronuciferine (6). The structures of these compounds were characterized by spectroscopic methods and comparison of physical properties with those reported in the literature. Among them, compounds 1 and 2 exhibited significant activity against human colon adenocarcinoma cell line (HT-29), with IC50 values of 0.70 and 1.61 µM, respectively, which were 8- and 3.5-fold higher than that of the reference anticancer drug, doxorubicin (IC50 5.63 µM). Moreover, compounds 1 and 2 displayed less cytotoxic activity against the non-cancerous HEK 239 cells with the IC50 values of 42.48 and 12.19 μM, respectively, whereas the cytotoxicity of doxorubicin against this cell line was 0.22 μM. The very potent cytotoxicity against HT-29 cell line and very high selectivity index (60.6-fold) of the alkaloid 1 is of particular significant; it could be considered as a promising structure lead for anti-colon cancer drug development. © 2017, Springer Science+Business Media, LLC, part of Springer Nature.
Subject(s)
Alkaloid
Armepavine
Cytotoxic agent
Doxorubicin
N methylcoclaurine
Neferine
Nelumbo nucifera extract
Nuciferine
O methylneferine
Pronuciferine
Unclassified drug
Article
Colon adenocarcinoma
Controlled study
Cytotoxicity
Drug isolation
Drug potency
Drug selectivity
Drug structure
HEK293 cell line
HT-29 cell line
Human
Human cell
IC50
Nelumbo nucifera
Nonhuman
Phytochemistry
Plant seed
Armepavine
Cytotoxic agent
Doxorubicin
N methylcoclaurine
Neferine
Nelumbo nucifera extract
Nuciferine
O methylneferine
Pronuciferine
Unclassified drug
Article
Colon adenocarcinoma
Controlled study
Cytotoxicity
Drug isolation
Drug potency
Drug selectivity
Drug structure
HEK293 cell line
HT-29 cell line
Human
Human cell
IC50
Nelumbo nucifera
Nonhuman
Phytochemistry
Plant seed