Publication:
A new second intermediate host and phylogenetic relationships based on the ITS2 sequence of Isoparorchis sp. (Digenea: Isoparorchiidae) in Thailand

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

0022149X

DOI

10.1017/S0022149X2100050X

Other identifier(s)

2-s2.0-85115923918

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Helminthology. Vol 95, No. (2021), p.286-292

Suggested Citation

Jaruboonyakorn P., Chontananarth T. A new second intermediate host and phylogenetic relationships based on the ITS2 sequence of Isoparorchis sp. (Digenea: Isoparorchiidae) in Thailand. Journal of Helminthology. Vol 95, No. (2021), p.286-292. doi:10.1017/S0022149X2100050X Retrieved from: https://hdl.handle.net/20.500.14740/8195

Title

A new second intermediate host and phylogenetic relationships based on the ITS2 sequence of Isoparorchis sp. (Digenea: Isoparorchiidae) in Thailand

Author(s)

Jaruboonyakorn P.
 Chontananarth T.

Abstract

The genus Isoparorchis (Family: Isoparorchiidae) contains trematodes infecting the air bladder of freshwater catfishes in Asia and Australia. Isoparorchis spp. rely on freshwater shrimps and fishes as intermediate hosts. There is limited information about parasitic infections in freshwater shrimp in Thailand, and Isoparorchis infection in an intermediate host in this country has never been reported. Thus, this study reports infections in freshwater shrimp (Macrobrachium lanchesteri and Caridina sp.), including overall prevalence, mean intensity, morphological characters and molecular analyses. The parasite specimens were analysed by studying their morphological characters, together with a molecular approach based on internal transcribed spacer 2 (ITS2) sequence data. The overall prevalence and mean intensity of Isoparorchis infections were 92% and 1.89, respectively. The metacercariae were identified as Isoparorchis sp. based on their morphological characters and supported by the comparison with published ITS2 sequences of Isoparorchis species. The phylogenetic studies based on the ITS2 region demonstrated that all of the Isoparorchis sp. specimens in this study are distinct from Isoparorchis species in previous reports. Moreover, we show for the first time that the freshwater shrimp M. lanchesteri serves as a second intermediate host of Isoparorchis sp. and we provide a morphological description and molecular characterization of Isoparorchis sp. metacercariae based on ITS2 sequence data to clarify the status of Isoparorchis sp. in Thailand. © The Author(s), 2021. Published by Cambridge University Press.

Subject(s)

Fresh water
 Genomic DNA
 Internal transcribed spacer 2
 Nucleotide
 Ribosome RNA
 RNA 28S
 Adult
 Animal experiment
 Animal model
 Animal tissue
 Article
 Caridina
 Comparative study
 Controlled study
 Digenea
 DNA extraction
 Intermediate host
 Isoparorchis
 Isoparorchis eurytremum
 Isoparorchis hypselobagri
 Isoparorchis infection
 Isoparorchis tandani
 Isoparorchis trisimilitubis
 Macrobrachium
 Macrobrachium lanchesteri
 Metacercaria
 Molecular biology
 Molecular phylogeny
 Morphological trait
 Nonhuman
 Nucleotide sequence
 Nucleotide sequence
 Parasite
 Parasitosis
 Prevalence
 Sequence analysis
 Shrimp
 Thailand
 Trematode

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URI

https://hdl.handle.net/20.500.14740/8195

Collections

Scopus: Year 1983-2021

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