Publication:
One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase

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Issued Date

2022

Resource Type

Article

Language

eng

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application/pdf

ISSN

23065354

DOI

10.3390/bioengineering9010017

Rights Holder(s)

Scopus

Bibliographic Citation

Bioengineering. Vol 9, No.1 (2022)

Suggested Citation

Sriariyanun M., Kitiborwornkul N., Tantayotai P., Rattanaporn K., Show P.-L. One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase. Bioengineering. Vol 9, No.1 (2022). doi:10.3390/bioengineering9010017 Retrieved from: https://hdl.handle.net/20.500.14740/10318

Title

One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase

Author(s)

Sriariyanun M.
 Kitiborwornkul N.
 Tantayotai P.
 Rattanaporn K.
 Show P.-L.

Abstract

Ionic liquid (IL) pretreatment of lignocellulose is an efficient method for the enhancement of enzymatic saccharification. However, the remaining residues of ILs deactivate cellulase, therefore making intensive biomass washing after pretreatment necessary. This study aimed to develop the one-pot process combining IL pretreatment and enzymatic saccharification by using low-toxic choline acetate ([Ch][OAc]) and IL-tolerant bacterial cellulases. Crude cellulases produced from saline soil inhabited Bacillus sp. CBD2 and Brevibacillus sp. CBD3 were tested under the influence of 0.5–2.0 M [Ch][OAc], which showed that their activities retained at more than 95%. However, [Ch][OAc] had toxicity to CBD2 and CBD3 cultures, in which only 32.85% and 12.88% were alive at 0.5 M [Ch][OAc]. Based on the specific enzyme activities, the sugar amounts produced from one-pot processes using 1 mg of CBD2 and CBD3 were higher than that of Celluclast 1.5 L by 2.0 and 4.5 times, respectively, suggesting their potential for further application in the biorefining process of value-added products. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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URI

https://hdl.handle.net/20.500.14740/10318

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Scopus: Year 2022

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