Publication:
Enzyme-free impedimetric biosensor-based molecularly imprinted polymer for selective determination of L-hydroxyproline

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

9565663

DOI

10.1016/j.bios.2021.113387

Other identifier(s)

2-s2.0-85107970643

Rights Holder(s)

Scopus

Bibliographic Citation

Biosensors and Bioelectronics. Vol 191, No. (2021)

Suggested Citation

Jesadabundit W., Jampasa S., Patarakul K., Siangproh W., Chailapakul O. Enzyme-free impedimetric biosensor-based molecularly imprinted polymer for selective determination of L-hydroxyproline. Biosensors and Bioelectronics. Vol 191, No. (2021). doi:10.1016/j.bios.2021.113387 Retrieved from: https://hdl.handle.net/20.500.14740/8151

Title

Enzyme-free impedimetric biosensor-based molecularly imprinted polymer for selective determination of L-hydroxyproline

Author(s)

Jesadabundit W.
 Jampasa S.
 Patarakul K.
 Siangproh W.
 Chailapakul O.

Abstract

This study first reported enzyme-free impedimetric biosensor-based molecularly imprinted polymers for selective and sensitive determination of L-hydroxyproline (L-hyp), a biomarker for the early diagnosis of bone diseases. In recent study, utilizing a single 3-aminophenylboronic acid (3-APBA) to create imprinted surfaces could result in a strong interaction and difficulty in removal of a template molecule. Hence, a mixture of monomer solution containing 3-APBA and o-phenylenediamine (OPD) in the presence of the L-hyp molecule was co-electropolymerized onto the screen-printed electrode using cyclic voltammetry (CV) to eradicate this mentioned limitation. The detection principle of this sensor is relied on alteration of mediator's charge transfer resistance (Rct) that could be obstructed by L-hyp occupied in imprinted surface. The successfully fabricated biosensor was explored by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and confocal scanning microscopy. Furthermore, the effect of polymer composition on the Rct response was systematically investigated. The result exhibited that the mixture of monomers could provide the highest change of Rct due to high selectivity from esterification of 3-APBA and from hydrogen bond of OPD surrounding the template. The sensor showed a significant increase in Rct in the presence of L-hyp, whereas no observable resistance change was detected in the absence thereof. The calibration curve was obtained in the range from 0.4 to 25 μg mL−1 with limits of detection (3SDblank/Slope) and quantification (10SDblank/Slope) of 0.13 and 0.42 μg mL−1, respectively. This biosensor exhibited high selectivity and sensitivity and was successfully applied to determine L-hyp in human serum samples with satisfactory results. © 2021 Elsevier B.V.

Subject(s)

Biosensors
 Bone
 Charge transfer
 Cyclic voltammetry
 Diagnosis
 Enzymes
 Fourier transform infrared spectroscopy
 Hydrogen bonds
 Mixtures
 Molecules
 Monomers
 Scanning electron microscopy
 3-Aminophenylboronic acid
 Bone disease
 Electrochemical-impedance spectroscopies
 High selectivity
 Impedimetric biosensors
 L hydroxyprolines
 Molecularly Imprinted Polymer
 Nonenzymatic sensor
 O-Phenylenediamine
 Selective determination
 Electrochemical impedance spectroscopy
 1,2 phenylenediamine
 Hydroxyproline
 Polymer
 Hydroxyproline
 Molecularly imprinted polymer
 Article
 Bone disease
 Calibration
 Confocal laser scanning microscopy
 Cyclic voltammetry
 Esterification
 Fourier transform infrared spectroscopy
 Human
 Hydrogen bond
 Impedance spectroscopy
 Limit of detection
 Molecular imprinting
 Normal human
 Scanning electron microscopy
 Electrochemical analysis
 Electrode
 Genetic procedures
 Biosensing Techniques
 Electrochemical Techniques
 Electrodes
 Humans
 Hydroxyproline
 Limit of Detection
 Molecular Imprinting
 Molecularly Imprinted Polymers

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URI

https://hdl.handle.net/20.500.14740/8151

Collections

Scopus: Year 1983-2021

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