Publication: Protective Effect and Mechanism of Fruit Extract of Aegle marmelos Against Amyloid-β Toxicity in a Transgenic Caenorhabditis elegans
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Issued Date
2020
Resource Type
File Type
application/pdf
ISSN
1934578X
Other identifier(s)
2-s2.0-85088094490
Rights Holder(s)
Scopus
Bibliographic Citation
Natural Product Communications. Vol 15, No.7 (2020)
Suggested Citation
Keowkase R., Kijmankongkul N., Sangtian W., Poomborplab S., Santa-Ardharnpreecha C., Weerapreeyakul N., Sitthithaworn W. Protective Effect and Mechanism of Fruit Extract of Aegle marmelos Against Amyloid-β Toxicity in a Transgenic Caenorhabditis elegans. Natural Product Communications. Vol 15, No.7 (2020). doi:10.1177/1934578X20933511 Retrieved from: https://hdl.handle.net/20.500.14740/4797
Abstract
Alzheimer’s disease (AD) is the most common form of dementia found in the elderly. AD is caused by the accumulation of toxic proteins including amyloid-β (Aβ). The purpose of this study was to investigate the effect of fruit extract of Aegle marmelos against Aβ toxicity in Caenorhabditis elegans. The fruit of A. marmelos has been used in a traditional Thai herb formula in fatigue patients recovering from illnesses such as fever and diarrhea. We used a transgenic C. elegans strain CL4176, which expresses the human Aβ42, to investigate the effects and the mechanisms of action of the extracts against Aβ toxicity. The extract of A. marmelos significantly delayed Aβ-induced paralysis. Aegle marmelos lost the ability to delay Aβ-induced paralysis in worms fed with daf-16 ribonucleic acid interference (RNAi) bacteria, but not in worms fed with hsf-1 and skin-1 RNAi bacteria. These results indicated that daf-16 transcription factor was required for A. marmelos-mediated delayed paralysis. Aegle marmelos enhanced the level of daf-16 gene. Taken together, these results indicated that A. marmelos reduced Aβ toxicity via the DAF-16-mediated cell signaling pathway. In addition, A. marmelos reduced toxic Aβ oligomers. Aegle marmelos also displayed antioxidative effect in in vivo as it enhanced resistance to paraquat-induced oxidative stress in wild type worms. All of the results suggested that A. marmelos can protect against Aβ-induced toxicity and can be a potential candidate for the prevention or treatment of AD. © The Author(s) 2020.
Subject(s)
Aegle marmelos extract
Amyloid beta protein[25-35]
Epigallocatechin gallate
Oligomer
Paraquat
Transcription factor DAF 16
Animal experiment
Animal model
Animal tissue
Antioxidant activity
Article
Caenorhabditis elegans
Controlled study
Drug effect
Drug mechanism
Fruit
High performance liquid chromatography
In vivo study
Neuroprotection
Nonhuman
Oxidative stress
Paralysis
Protein expression
RNA interference
Signal transduction
Transgenic animal
Wild type
Amyloid beta protein[25-35]
Epigallocatechin gallate
Oligomer
Paraquat
Transcription factor DAF 16
Animal experiment
Animal model
Animal tissue
Antioxidant activity
Article
Caenorhabditis elegans
Controlled study
Drug effect
Drug mechanism
Fruit
High performance liquid chromatography
In vivo study
Neuroprotection
Nonhuman
Oxidative stress
Paralysis
Protein expression
RNA interference
Signal transduction
Transgenic animal
Wild type