Publication:
Terrein induces apoptosis in HeLa human cervical carcinoma cells through p53 and ERK regulation

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

1021335X

DOI

10.3892/or.2013.2288

Other identifier(s)

2-s2.0-84874728121

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Oncology Reports. Vol 29, No.4 (2013), p.1600-1608

Suggested Citation

Porameesanaporn Y., Uthaisang-Tanechpongtamb W., Jarintanan F., Jongrungruangchok S., Wongsatayanon B.T. Terrein induces apoptosis in HeLa human cervical carcinoma cells through p53 and ERK regulation. Oncology Reports. Vol 29, No.4 (2013), p.1600-1608. doi:10.3892/or.2013.2288 Retrieved from: https://hdl.handle.net/20.500.14740/6690

Title

Terrein induces apoptosis in HeLa human cervical carcinoma cells through p53 and ERK regulation

Author(s)

Porameesanaporn Y.
 Uthaisang-Tanechpongtamb W.
 Jarintanan F.
 Jongrungruangchok S.
 Wongsatayanon B.T.

Abstract

Terrein, a fungal metabolite derived from Aspergillus terreus, has been shown to have a variety of biological activities in human cells including inhibition of melanogenesis, as well as anti-inflammatory, antioxidant and anticancer properties. In the present study, terrein was shown to have marked anticancer activity on HeLa human cervical carcinoma cells. Terrein exhibited inhibition of proliferation within the same ranges for other cancer cell types with an IC50 at 0.29 mM. The growth inhibition that induced cell death was via apoptosis mechanisms. Chromatin condensation was observed using the Hoechst 33342 stain, a DNA-specific dye. The increase of DNA fragmentation or the sub-G0 peak was also detected by flow cytometry. The signaling used by terrein to induce apoptosis was via the death-receptor and mitochondrial pathways; the cleavage of specific fluorogenic substrates by caspase-3, -8 and -9 activities are clearly demonstrated. The mitochondria were damaged as demonstrated by the decrease of the red/green ratio of the JC-1 staining and the increase of the Bax/Bcl-2 expression ratio. Further analysis of the upstream signaling by the quantitative real-time polymerase chain reaction showed that p53, p21 and ERK were upregulated which indicates the importance of their roles on terrein signaling. This study is the first to show that terrein has an effect on the anticancer properties in cervical cancer cells by inducing apoptosis through p53 and ERK regulation. Our data may help expand the function of the terrein compound and may also aid in the discovery of new anticancer agents. © 2013 Spandidos Publications Ltd. All rights reserved.

Subject(s)

Caspase 3
 Caspase 8
 Caspase 9
 Death receptor
 Hoe 33342
 Mitogen activated protein kinase
 Protein Bax
 Protein bcl 2
 Protein p21
 Protein p53
 Terrein
 Antineoplastic activity
 Apoptosis
 Article
 Cancer cell
 Cancer inhibition
 Cell cycle G0 phase
 Cell death
 Cell proliferation
 Chromatin condensation
 Controlled study
 DNA fragmentation
 Drug cytotoxicity
 Drug development
 Drug mechanism
 Drug structure
 Enzyme activity
 Enzyme substrate
 Flow cytometry
 HeLa cell
 Human
 Human cell
 IC 50
 Mitochondrion
 Priority journal
 Protein expression
 Real time polymerase chain reaction
 Signal transduction
 Upregulation
 Uterine cervix carcinoma
 Antineoplastic Agents
 Apoptosis
 Aspergillus
 Carcinoma
 Cell Proliferation
 Cyclopentanes
 Female
 Gene Expression Regulation, Neoplastic
 HeLa Cells
 Humans
 MAP Kinase Signaling System
 Mitochondria
 Tumor Suppressor Protein p53
 Uterine Cervical Neoplasms

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URI

https://hdl.handle.net/20.500.14740/6690

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Scopus: Year 1983-2021

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