Publication:
Generation of two human iPSC lines (MDCUi001-A and MDCUi001-B) from dermal fibroblasts of a Thai patient with X-linked osteogenesis imperfecta using integration-free Sendai virus

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

18735061

DOI

10.1016/j.scr.2019.101493

Other identifier(s)

2-s2.0-85069590379

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Stem Cell Research. Vol 39, (2019)

Suggested Citation

Tongkobpetch S., Rungsiwiwut R., Pruksananonda K., Suphapeetiporn K., Shotelersuk V. Generation of two human iPSC lines (MDCUi001-A and MDCUi001-B) from dermal fibroblasts of a Thai patient with X-linked osteogenesis imperfecta using integration-free Sendai virus. Stem Cell Research. Vol 39, (2019). doi:10.1016/j.scr.2019.101493 Retrieved from: https://hdl.handle.net/20.500.14740/5190

Title

Generation of two human iPSC lines (MDCUi001-A and MDCUi001-B) from dermal fibroblasts of a Thai patient with X-linked osteogenesis imperfecta using integration-free Sendai virus

Author(s)

Tongkobpetch S.
 Rungsiwiwut R.
 Pruksananonda K.
 Suphapeetiporn K.
 Shotelersuk V.

Abstract

Two clones of human induced pluripotent stem cells (iPSCs) were generated from dermal fibroblasts isolated from a one-year-old Thai patient with X-linked osteogenesis imperfecta. The patient harbored a mutation, p.N459S, in the MBTPS2 gene. The cells were reprogrammed using an integration-free Sendai virus containing KLF4, c-MYC, OCT4 and SOX2. Both of the established iPSC lines (MDCUi001-A and MDCUi001-B) maintained normal karyotype, expressed pluripotent markers and differentiated into all three germ layers. © 2019

Subject(s)

Afp1 protein
 Alpha smooth muscle actin
 Beta tubulin
 Cell marker
 Genomic DNA
 Glyceraldehyde 3 phosphate dehydrogenase
 Kruppel like factor 4
 Membrane bound transcription factor peptidase site 2
 Myc protein
 Octamer transcription factor 4
 Peptidase
 Rex1 protein
 Tra 1 60 protein
 Tra 1 81 protein
 Transcription factor
 Transcription factor NANOG
 Transcription factor Sox2
 Unclassified drug
 Kruppel like factor
 Kruppel like factor 4
 Myc protein
 MYC protein, human
 SOX2 protein, human
 Transcription factor Sox
 Article
 Cell clone
 Cell differentiation
 Cell reprogramming technique
 Child
 Controlled study
 Ectoderm
 Embryoid body
 Endoderm
 Human
 Human cell
 In vitro study
 Induced pluripotent stem cell
 Karyotype
 Male
 Mdcui001 a cell line
 Mdcui001 b cell line
 Mesoderm
 Nonhuman
 Nuclear reprogramming
 Osteogenesis imperfecta
 Pluripotent stem cell line
 Point mutation
 Preschool child
 Priority journal
 Sendai virus
 Short tandem repeat
 Skin fibroblast
 Thai (people)
 Cell line
 Cytology
 Fibroblast
 Genetics
 Induced pluripotent stem cell
 Metabolism
 Osteogenesis imperfecta
 Physiology
 Sendai virus
 Thailand
 Cell Differentiation
 Cell Line
 Fibroblasts
 Humans
 Induced Pluripotent Stem Cells
 Karyotype
 Kruppel-Like Transcription Factors
 Male
 Osteogenesis Imperfecta
 Proto-Oncogene Proteins c-myc
 Sendai virus
 SOXB1 Transcription Factors
 Thailand

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URI

https://hdl.handle.net/20.500.14740/5190

Collections

Scopus: Year 1983-2021

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