Publication: Sequential injection system for simultaneous determination of sucrose and phosphate in cola drinks using paired emitter-detector diode sensor
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Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
399140
Other identifier(s)
2-s2.0-84879108107
Rights Holder(s)
Scopus
Bibliographic Citation
Talanta. Vol 115, No. (2013), p.361-366
Suggested Citation
Saetear P., Khamtau K., Ratanawimarnwong N., Sereenonchai K., Nacapricha D. Sequential injection system for simultaneous determination of sucrose and phosphate in cola drinks using paired emitter-detector diode sensor. Talanta. Vol 115, No. (2013), p.361-366. doi:10.1016/j.talanta.2013.05.051 Retrieved from: https://hdl.handle.net/20.500.14740/6831
Abstract
This work presents the simultaneous determination of sucrose and phosphate by using sequential injection (SI) system with a low cost paired emitter-detector diode (PEDD) light sensor. The PEDD uses two 890 nm LEDs. Measurement of sucrose in Brix unit was carried out based on the detection of light refraction occurring at the liquid interface (the schlieren effect) between the sucrose solution and water. Phosphate was measured from the formation of calcium phosphate with turbidimetric detection. With careful design of the loading sequence and volume (sample - precipitating reagent - sample), simultaneous detection of sucrose and phosphate was accomplished with the single PEDD detector. At the optimized condition, linear calibrations from 1 to 7 Brix sucrose and from 50 to 200 mg PO43- L-1 were obtained. Good precision at lower than 2% RSD (n=10) for both analytes with satisfactory throughput of 21 injections h-1 was achieved. The method was successfully applied for the determination of sucrose and phosphate in cola drinks. The proposed method is readily applicable for automation and is found to be an alternative method to conventional procedures for on-line quality control process in cola drink industry. © 2013 Published by Elsevier B.V.All rights reserved.