Publication: Apoptosis induction in breast cancer cells by cowanin
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Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-85075014703
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S7-S12
Suggested Citation
Chowchaikong N., Nilwarangoon S., Tanjapatkul N., Laphookhieo S., Watanapokasin R. Apoptosis induction in breast cancer cells by cowanin. Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S7-S12. Retrieved from: https://hdl.handle.net/20.500.14740/4330
Abstract
Background: Breast cancer is a common cancer occurs in women worldwide. In Thailand and United States, it is the first and second leading cause of cancer-related death among females, respectively. Therefore, a novel bioactive compound with high selectivity for cancer cell death is important in cancer research. Objective: To investigate the effect of cowanin on anti-proliferation and apoptosis induction of breast cancer cell, MDA-MB-468. Material and Method: MTT assay was used for cell proliferation and viability assay. Nuclear morphological changes and mitochondrial membrane potential was determined by Hoechst 33342 staining and JC-1 staining, respectively. Results: Cowanin showed anti-proliferation and cell viability reduction in MDA-MB-468 cells in a time- and dose-dependent manner with IC50 value of 16.27+0.88 μg/mL. Furthermore, cowanin induced chromatin condensation, apoptotic bodies and mitochondrial membrane dysfunction in MDA-MB-468 cells. Conclusion: Cowanin showed anti-proliferation and apoptosis induction in MDA-MB-468 cells. These results are basic knowledge for developing the chemotherapeutic agent for breast cancer in the future. © 2017 Medical Association of Thailand. All rights reserved.