Publication: Biodiversity of thermotolerant Bacillus sp. producing biosurfactants, biocatalysts, and antimicrobial agents
0
0
Issued Date
2008
Resource Type
File Type
application/pdf
ISSN
15131874
Other identifier(s)
2-s2.0-58649083927
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
ScienceAsia. Vol 34, No.4 (2008), p.424-431
Suggested Citation
Pakpitcharoen A., Potivejkul K., Kanjanavas P., Areekit S., Chansiri K. Biodiversity of thermotolerant Bacillus sp. producing biosurfactants, biocatalysts, and antimicrobial agents. ScienceAsia. Vol 34, No.4 (2008), p.424-431. doi:10.2306/scienceasia1513-1874.2008.34.424 Retrieved from: https://hdl.handle.net/20.500.14740/4169
Abstract
Thermotolerant bacteria isolated from soil and water samples taken from 76 hot springs in Thailand were investigated for their biosurfactant, biocatalytic, and antimicrobial properties. DNA samples purified from 148 pure isolates were PCR amplified using primers specific for the 16S rDNA hypervariable region of the genus Bacillus. The DNAPAR phylogenetic tree clearly demonstrated that these isolates were related to Bacillus niacini, B. korensis, B. firmus, B. flexus, B. megaterium, B. pumilus, B. licheniformis, B. subtilis, B. fusiformis, Oceanobacillus picturae, Anoxybacillus gonensis, Aneurinibacillus thermoaerophilus, and Anoxybacillus flavithermus. Determination of their biosurfactant properties using the oil spreading technique indicated that B. megaterium (SR8.5), B. licheniformis (CM8.2), and B. pumilus (SR4.4) possessed the highest biosurfactant activity. In addition, lipase, cellulase, and xylanase activities were detected from B. megaterium (CP1, SR8.5), B. pumilus (PR3.2), and B. licheniformis (CM1.1, LP3, MH11.3, LP3, NN2.3, PR2.3, PR2.2, PT1.2). While B. subtilis (CM3.3, CM14.1) had the highest cellulase activity, most isolates showed either undetectable or relatively low lipase and xylanase activities.