Publication:
In vitro modulation of tumor necrosis factor α production in THP-1 cells by lactic acid bacteria isolated from healthy human infants

0
0

Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

10759964

DOI

10.1016/j.anaerobe.2015.03.002

Other identifier(s)

2-s2.0-84924532766

Rights Holder(s)

Scopus

Bibliographic Citation

Anaerobe. Vol 33, (2015), p.109-116

Suggested Citation

Ladda B., Theparee T., Chimchang J., Tanasupawat S., Taweechotipatr M. In vitro modulation of tumor necrosis factor α production in THP-1 cells by lactic acid bacteria isolated from healthy human infants. Anaerobe. Vol 33, (2015), p.109-116. doi:10.1016/j.anaerobe.2015.03.002 Retrieved from: https://hdl.handle.net/20.500.14740/6136

Title

In vitro modulation of tumor necrosis factor α production in THP-1 cells by lactic acid bacteria isolated from healthy human infants

Author(s)

Ladda B.
 Theparee T.
 Chimchang J.
 Tanasupawat S.
 Taweechotipatr M.

Abstract

The human microbiota is a source of probiotics capable of modulating the host immune system. In this study, we collected fecal samples from 100 healthy infants and isolated lactic acid bacteria which were screened for immune modulating effects on tumor necrosis factor α (TNF-α) production. Cell-free culture supernatants from 26 isolates were able to decrease TNF-α production invitro and three of the isolates were selected as candidate probiotics (MSMC39-1, MSMC39-3, MSMC57-1). These isolates were identified using 16S ribosomal DNA sequencing as Lactobacillus paracasei, Lactobacillus casei, and Weissella confusa respectively. All three isolates were acid tolerant and bile tolerant to pH 3.0 and 4% bile respectively. Preparations of cell-free culture supernatants were processed and tested, and revealed that cell-free culture supernatants of isolates L.paracasei MSMC39-1, L.casei MSMC39-3, and W.confusa MSMC57-1 decreased the production of TNF-α significantly and were heat resistant. Only L.paracasei MSMC39-1 supernatant was proteinase-K sensitive. The effects of viable bacteria, heat-killed bacteria, and sonicated bacteria were compared. The heat-killed preparations of isolate W.confusa MSMC57-1 decreased the production of TNF-α. Sonicated cell preparations did not significantly alter TNF-α production. For isolates L.paracasei MSMC39-1 and L.casei MSMC39-3, this suggests that a substance in the cell-free culture supernatant may be responsible for invitro cytokine modulation. © 2015 Elsevier Ltd.

Subject(s)

DNA 16S
 Probiotic agent
 Tumor necrosis factor alpha
 Probiotic agent
 Tumor necrosis factor
 Acid tolerance
 Article
 Bacterial strain
 Bacterium culture
 Bacterium isolate
 Cell free system
 Cytokine production
 DNA sequence
 Embryo
 Feces analysis
 Heating
 Human
 Immunomodulation
 Klebsiella pneumoniae
 Lactic acid bacterium
 Lactobacillus casei
 Lactobacillus paracasei
 Monocyte
 Nonhuman
 Priority journal
 Weissella
 Weissella confusa
 Adaptation
 Biosynthesis
 Cell culture
 Cell line
 Feces
 Immunology
 Immunomodulation
 Infant
 Intestine mucosa
 Isolation and purification
 Lactobacillus
 Metabolism
 Microbiology
 Newborn
 Physiology
 Lactobacillus casei
 Lactobacillus paracasei
 Weissella confusa
 Adaptation, Physiological
 Cell Line
 Cells, Cultured
 Feces
 Humans
 Immunomodulation
 Infant
 Infant, Newborn
 Intestinal Mucosa
 Lactobacillus
 Monocytes
 Probiotics
 Tumor Necrosis Factor-alpha

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6136

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By