Publication:
Possible mechanisms of vasorelaxation for 5,7-dimethoxyflavone from Kaempferia parviflora in the rat aorta

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

0951418X

DOI

10.1002/ptr.3164

Other identifier(s)

2-s2.0-78649435662

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Phytotherapy Research. Vol 24, No.10 (2010), p.1520-1525

Suggested Citation

Tep-Areenan P., Sawasdee P., Randall M. Possible mechanisms of vasorelaxation for 5,7-dimethoxyflavone from Kaempferia parviflora in the rat aorta. Phytotherapy Research. Vol 24, No.10 (2010), p.1520-1525. doi:10.1002/ptr.3164 Retrieved from: https://hdl.handle.net/20.500.14740/7541

Title

Possible mechanisms of vasorelaxation for 5,7-dimethoxyflavone from Kaempferia parviflora in the rat aorta

Author(s)

Tep-Areenan P.
 Sawasdee P.
 Randall M.

Abstract

The present study investigated the vascular effects of 5,7-dimethoxyflavone (DMF), isolated from the rhizomes of Kaempferia parviflora (KP), on rat isolated aortic rings and its possible mechanisms. DMF (1-100 μm) caused concentration-dependent relaxations in aortic rings precontracted with methoxamine. This effect was significantly reduced by removal of the endothelium, and after pretreatment with NG-nitro-L-arginine methyl ester (L-NAME, 300 μm), indomethacin (10 μm) and 1H-[1,2,4]oxadiazolo-[4, 3-a]quinoxalin-1-one (ODQ, 10 μm), but not 9-(tetrahydro-2-furanyl)-9H- purine-6-amine (SQ22536, 100 μm). Relaxant responses to DMF were significantly inhibited by high KCl (60 mm) in both endothelium-intact and -denuded rings. In addition, the relaxations to DMF were significantly reduced by pretreatment with tetraethylammonium (TEA, 5 mm), glibenclamide (10 μm), 4-aminopyridine (1 mm) or barium chloride (10 μm). Preincubation with DMF (10 and 100 μm) for 30 min significantly inhibited the contractile responses to CaCl2 in a Ca2+-free, high K+ buffer. The present study demonstrated that DMF causes endothelium-dependent relaxation that is partly mediated by NO-cGMP and cyclooxygenase pathways. Interestingly, DMF-induced responses are mainly due to increasing K+ efflux, and inhibition of Ca2+ influx from the extracellular space. The vasodilator effects of DMF provide experimental support for the potential use of KP as a medical plant in the treatment of cardiovascular diseases. © 2010 John Wiley & Sons, Ltd.

Subject(s)

1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one
 4 aminopyridine
 5,7 dimethoxyflavone
 Barium chloride
 Buffer
 Calcium chloride
 Flavone
 Glibenclamide
 Indometacin
 Methoxamine
 N(g) nitroarginine methyl ester
 Potassium chloride
 Tetrylammonium
 Unclassified drug
 Animal cell
 Animal experiment
 Article
 Calcium transport
 Cardiovascular disease
 Controlled study
 Endothelium
 Extracellular space
 Male
 Medicinal plant
 Nonhuman
 Potassium transport
 Rat
 Rhizome
 Vascular ring
 Vasodilatation
 Animals
 Aorta
 Calcium
 Cyclic GMP
 Flavonoids
 Male
 Nitric Oxide
 Rats
 Rats, Wistar
 Rhizome
 Vasodilation
 Zingiberaceae
 Kaempferia parviflora
 Rattus

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URI

https://hdl.handle.net/20.500.14740/7541

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