Publication:
Involvement of reactive oxygen species and stress-activated MAPKs in satratoxin H-induced apoptosis

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Issued Date

2005

Resource Type

Article

File Type

application/pdf

ISSN

142999

DOI

10.1016/j.ejphar.2004.11.046

Other identifier(s)

2-s2.0-19944431322

Rights Holder(s)

Scopus

Bibliographic Citation

European Journal of Pharmacology. Vol 507, (2005), p.239-246

Suggested Citation

Nusuetrong P., Yoshida M., Tanitsu M.-A., Kikuchi H., Mizugaki M., Shimazu K.-I., Pengsuparp T., Meksuriyen D., Oshima Y., Nakahata N. Involvement of reactive oxygen species and stress-activated MAPKs in satratoxin H-induced apoptosis. European Journal of Pharmacology. Vol 507, (2005), p.239-246. doi:10.1016/j.ejphar.2004.11.046 Retrieved from: https://hdl.handle.net/20.500.14740/6177

Title

Involvement of reactive oxygen species and stress-activated MAPKs in satratoxin H-induced apoptosis

Author(s)

Nusuetrong P.
 Yoshida M.
 Tanitsu M.-A.
 Kikuchi H.
 Mizugaki M.
 Shimazu K.-I.
 Pengsuparp T.
 Meksuriyen D.
 Oshima Y.
 Nakahata N.

Abstract

Satratoxins, members of the trichothecene mycotoxin family, have been known to be harmful to health. However, the mechanisms underlying the toxicity still remain unclear. The present study is undertaken to elucidate the mechanisms of the satratoxin H-induced cytotoxicity in PC12 cells. Satratoxin H caused cytotoxicity, which was reflected from apoptosis determined by chromatin staining and flow cytometry. Satratoxin H stimulated the phosphorylation of extracellular signal-regulated kinase (ERK), p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK). Pre-incubation with SB203580, a p38 MAPK inhibitor, or SP600125, a JNK inhibitor, but not PD98059, an ERK inhibitor, reduced satratoxin-induced cytotoxicity. Co-incubation of cells with glutathione, N-acetyl-l-cysteine or glutathione reductase inhibited cytotoxicity and the phosphorylation of p38 MAPK induced by satratoxin H. Our data suggest that satratoxin H-induced apoptosis in PC12 cells is dependent on the activation of p38 MAPK/JNK and the increase in reactive oxygen species. © 2004 Elsevier B.V. All rights reserved.

Subject(s)

2 (2 amino 3 methoxyphenyl)chromone
 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole
 Acetylcysteine
 Anthra[1,9 cd]pyrazol 6(2h) one
 Glutathione
 Glutathione reductase
 Mitogen activated protein kinase
 Mitogen activated protein kinase p38
 Mycotoxin
 Reactive oxygen metabolite
 Satratoxin h
 Stress activated protein kinase
 Unclassified drug
 Animal cell
 Apoptosis
 Article
 Cell stimulation
 Cell strain
 Chromatin structure
 Coculture
 Controlled study
 Cytopathogenic effect
 Drug inhibition
 Enzyme activation
 Flow cytometry
 Nonhuman
 Priority journal
 Protein phosphorylation
 Rat
 Staining
 Animals
 Apoptosis
 Cell Survival
 Dose-Response Relationship, Drug
 Mitogen-Activated Protein Kinases
 Mycotoxins
 PC12 Cells
 Rats
 Reactive Oxygen Species
 Stress
 Trichothecenes

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URI

https://hdl.handle.net/20.500.14740/6177

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Scopus: Year 1983-2021

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