Publication:
Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii

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Issued Date

2014

Resource Type

Article

File Type

application/pdf

ISSN

10504648

DOI

10.1016/j.fsi.2013.12.025

Other identifier(s)

2-s2.0-84893204687

Rights Holder(s)

Scopus

Bibliographic Citation

Fish and Shellfish Immunology. Vol 36, No.2 (2014), p.552-562

Suggested Citation

Srisuk C., Longyant S., Senapin S., Sithigorngul P., Chaivisuthangkura P. Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii. Fish and Shellfish Immunology. Vol 36, No.2 (2014), p.552-562. doi:10.1016/j.fsi.2013.12.025 Retrieved from: https://hdl.handle.net/20.500.14740/6395

Title

Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii

Author(s)

Srisuk C.
 Longyant S.
 Senapin S.
 Sithigorngul P.
 Chaivisuthangkura P.

Abstract

Toll receptors are cell surface molecules acting as pattern recognition receptors (PRRs) that have been implicated in the signaling pathway of innate immune responses. In this study, the full-length cDNA of a Toll receptor gene of Macrobrachium rosenbergii, designated MrToll, was successfully isolated using designed degenerate primers and the rapid amplification of cDNA ends (RACE). The MrToll gene sequence contained an open reading frame (ORF) of 2799 nucleotides encoding a protein of 932 amino acid residues. The protein contained distinct structural motifs of the Toll-like receptor (TLR) family, including an extracellular domain containing 15 leucine-rich repeats (LRRs), a transmembrane segment of 23 amino acids, and a cytoplasmic Toll/interleukin-1R (TIR) domain of 139 residues. Phylogenetic analysis revealed that MrToll and Toll receptor of Marsupenaeus japonicus (MjToll) evolved closely. However, the MrToll ORF demonstrated only 48-49% identity with shrimp Toll1, suggesting that MrToll isolated from a palaemonid shrimp might belong to a novel class of Toll receptors in shrimp. The transcripts of the MrToll gene were constitutively expressed in various tissues, with high levels in hemocytes, the stomach and muscle. A reverse transcriptase PCR assay demonstrated that the expression patterns of MrToll were distinctly modulated after Aeromonas caviae stimulation, with significant enhancement at 3-12h post-challenge and a decline to basal levels at 24h post-challenge. In addition, when MrToll-silenced shrimp were challenged with A.caviae, there was a significant increase in mortality and bacterial CFU counts. These results suggest that MrToll might be involved in host innate defense, especially against the pathogen A.caviae. © 2014 Elsevier Ltd.

Subject(s)

Arthropod protein
 Toll like receptor
 Aeromonas punctata
 Amino acid sequence
 Animal
 Article
 Chemistry
 Classification
 Gene expression regulation
 Genetics
 Immunology
 Innate immunity
 Macrobrachium rosenbergii
 Metabolism
 Microbiology
 Molecular genetics
 Nucleotide sequence
 Palaemonidae
 Phylogeny
 Physiology
 RNA interference
 Sequence alignment
 Toll receptor
 Aeromonas caviae
 Innate immunity
 Macrobrachium rosenbergii
 RNA interference
 Toll receptor
 Aeromonas caviae
 Amino Acid Sequence
 Animals
 Arthropod Proteins
 Base Sequence
 Gene Expression Regulation
 Immunity, Innate
 Molecular Sequence Data
 Palaemonidae
 Phylogeny
 RNA Interference
 Sequence Alignment
 Toll-Like Receptors

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URI

https://hdl.handle.net/20.500.14740/6395

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Scopus: Year 1983-2021

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