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Evaluation of real-time polymerase chain reaction for detection of the 16s ribosomal RNA gene of mycobacterium tuberculosis and the diagnosis of cervical tuberculous lymphadenitis in a country with a high tuberculosis incidence

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

10584838

DOI

10.1093/cid/cis401

Other identifier(s)

2-s2.0-84863924847

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Clinical Infectious Diseases. Vol 55, No.3 (2012), p.313-321

Suggested Citation

Linasmita P., Srisangkaew S., Wongsuk T., Bhongmakapat T., Watcharananan S.P. Evaluation of real-time polymerase chain reaction for detection of the 16s ribosomal RNA gene of mycobacterium tuberculosis and the diagnosis of cervical tuberculous lymphadenitis in a country with a high tuberculosis incidence. Clinical Infectious Diseases. Vol 55, No.3 (2012), p.313-321. doi:10.1093/cid/cis401 Retrieved from: https://hdl.handle.net/20.500.14740/6990

Title

Evaluation of real-time polymerase chain reaction for detection of the 16s ribosomal RNA gene of mycobacterium tuberculosis and the diagnosis of cervical tuberculous lymphadenitis in a country with a high tuberculosis incidence

Author(s)

Linasmita P.
 Srisangkaew S.
 Wongsuk T.
 Bhongmakapat T.
 Watcharananan S.P.

Abstract

Background.Tuberculous lymphadenitis (TBL) is the most common form of extrapulmonary tuberculosis. Currently, the standard diagnostic test for TBL is culture, which takes more than several weeks to yield results. We studied a real-time polymerase chain reaction (PCR) for rapid detection of Mycobacterium tuberculosis in cervical lymph node specimens obtained from patients in a country where the tuberculosis incidence is high.Methods.Patients with cervical lymphadenopathy were prospectively enrolled between April 2009 and March 2010. Clinical specimens obtained through fine-needle aspiration (FNA) and excisional biopsy were tested for M. tuberculosis by the COBAS TaqMan MTB Test, a real-time PCR assay for detecting the 16S ribosomal RNA gene of M. tuberculosis. Mycobacterial culture and histopathological findings from tissue biopsy specimens were used as a reference standard for sensitivity and specificity calculations.Results.Of 73 patients, 41 received a diagnosis of TBL. For biopsy specimens, the sensitivity of real-time PCR was 63.4, and the specificity was 96.9. For FNA specimens, the sensitivity was 17.1, and the specificity was 100. The sensitivity of real-time PCR of biopsy specimens was comparable to that of tissue culture but significant lower than that of histopathological examination (P <. 01).Conclusions.Real-time PCR did not increase the yield for rapid diagnosis of TBL. © 2012 The Author.

Subject(s)

RNA 16S
 Tuberculostatic agent
 Adult
 Article
 Aspiration biopsy
 Cervical lymph node
 Cross-sectional study
 Female
 Histopathology
 Human
 Human tissue
 Lymph node biopsy
 Major clinical study
 Male
 Mycobacterium tuberculosis
 Priority journal
 Prospective study
 Real time polymerase chain reaction
 Sensitivity and specificity
 Tuberculous lymphadenitis
 Adult
 Bacteriological Techniques
 Biopsy
 Biopsy, Fine-Needle
 Cross-Sectional Studies
 Female
 Histocytochemistry
 Humans
 Incidence
 Male
 Middle Aged
 Molecular Diagnostic Techniques
 Mycobacterium tuberculosis
 Prospective Studies
 Real-Time Polymerase Chain Reaction
 RNA, Bacterial
 RNA, Ribosomal, 16S
 Sensitivity and Specificity
 Tuberculosis, Lymph Node

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URI

https://hdl.handle.net/20.500.14740/6990

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Scopus: Year 1983-2021

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