Publication:
Pummelo protects doxorubicin-induced cardiac cell death by reducing oxidative stress, modifying glutathione transferase expression, and preventing cellular senescence

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

1741427X

DOI

10.1155/2013/254835

Other identifier(s)

2-s2.0-84874547456

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Evidence-based Complementary and Alternative Medicine. Vol 2013, No. (2013), p.-

Suggested Citation

Chularojmontri L., Gerdprasert O., Wattanapitayakul S.K. Pummelo protects doxorubicin-induced cardiac cell death by reducing oxidative stress, modifying glutathione transferase expression, and preventing cellular senescence. Evidence-based Complementary and Alternative Medicine. Vol 2013, No. (2013), p.-. doi:10.1155/2013/254835 Retrieved from: https://hdl.handle.net/20.500.14740/6696

Title

Pummelo protects doxorubicin-induced cardiac cell death by reducing oxidative stress, modifying glutathione transferase expression, and preventing cellular senescence

Author(s)

Chularojmontri L.
 Gerdprasert O.
 Wattanapitayakul S.K.

Abstract

Citrus flavonoids have been shown to reduce cardiovascular disease (CVD) risks prominently due to their antioxidant effects. Here we investigated the protective effect of pummelo (Citrus maxima, CM) fruit juice in rat cardiac H9c2 cells against doxorubicin (DOX-) induced cytotoxicity. Four antioxidant compositions (ascorbic acid, hesperidin, naringin, and gallic acid) were determined by HPLC. CM significantly increased cardiac cell survival from DOX toxicity as evaluated by MTT assay. Reduction of cellular oxidative stress was monitored by the formation of DCF fluorescent product and total glutathione (GSH) levels. The changes in glutathione-S-transferase (GST) activity and expression were determined by enzyme activity assay and Western blot analysis, respectively. Influence of CM on senescence-associated β-galactosidase activity (SA-β-gal) was also determined. The mechanisms of cytoprotection involved reduction of intracellular oxidative stress, maintaining GSH availability, and enhanced GST enzyme activity and expression. DOX-induced cellular senescence was also attenuated by long-term CM treatment. Thus, CM fruit juice can be promoted as functional fruit to protect cells from oxidative cell death, enhance the phase II GSTP enzyme activity, and decrease senescence phenotype population induced by cardiotoxic agent such as DOX. © 2013 L. Chularojmontri et al.

Subject(s)

3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
 Ascorbic acid
 Aurantiin
 Beta galactosidase
 Dichlorodihydrofluorescein diacetate
 Doxorubicin
 Gallic acid
 Glutathione
 Glutathione transferase
 Hesperidin
 Plant extract
 Pummelo extract
 Reactive oxygen metabolite
 Senescence associated beta galactosidase
 Unclassified drug
 Animal cell
 Antioxidant activity
 Article
 Cell aging
 Cell death
 Cell protection
 Cell survival
 Cell viability
 Chemical composition
 Controlled study
 Cytotoxicity
 Drug determination
 Drug mechanism
 Enzyme activity
 Enzyme assay
 Fruit juice
 Heart muscle cell
 High performance liquid chromatography
 Nonhuman
 Oxidative stress
 Priority journal
 Protein expression
 Pummelo
 Pummelo fruit juice
 Western blotting

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URI

https://hdl.handle.net/20.500.14740/6696

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Scopus: Year 1983-2021

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