Publication:
Complete nucleotide sequence and genomic organization of hepatopancreatic parvovirus (HPV) of Penaeus monodon

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Issued Date

2006

Resource Type

Article

File Type

application/pdf

ISSN

426822

DOI

10.1016/j.virol.2005.06.052

Other identifier(s)

2-s2.0-33644823974

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Virology. Vol 346, No.2 (2006), p.266-277

Suggested Citation

Sukhumsirichart W., Attasart P., Boonsaeng V., Panyim S. Complete nucleotide sequence and genomic organization of hepatopancreatic parvovirus (HPV) of Penaeus monodon. Virology. Vol 346, No.2 (2006), p.266-277. doi:10.1016/j.virol.2005.06.052 Retrieved from: https://hdl.handle.net/20.500.14740/5872

Title

Complete nucleotide sequence and genomic organization of hepatopancreatic parvovirus (HPV) of Penaeus monodon

Author(s)

Sukhumsirichart W.
 Attasart P.
 Boonsaeng V.
 Panyim S.

Abstract

We have determined the genome of hepatopancreatic parvovirus (HPV), a minus, single-stranded DNA virus isolated from infected Penaeus monodon in Thailand. Its genome consisted of 6321 nucleotides, representing three large open reading frames (ORFs) and two non-coding termini. The left (ORF1), mid (ORF2), and right (ORF3) ORFs on the complementary (plus) strand may code for 428, 579, and 818 amino acids, equivalent to 50, 68, and 92 kDa, respectively. The 5′ and 3′ ends of viral genome contained hairpin-like structure length of approximately 222 and 215 bp, respectively. No inverted terminal repeat (ITR) was detected. The ORF2 contained conserved replication initiator motif, NTP-binding and helicase domain similar to NS-1 of other parvoviruses. Therefore, it most likely encoded the major nonstructural protein (NS-1). The ORF1 encoded putative nonstructural protein-2 (NS-2) with unknown function. The ORF3 of the HPV genome encoded a capsid protein (VP) of approximately 92 kDa. This may be later cleaved after arginine residue to produce a 57-kDa structural protein. A phylogenetic tree based on conserved amino acid sequences (119 aa) revealed that it is closely related to Brevidensoviruses, which are shrimp parvovirus (IHHNV) and mosquito densoviruses (AaeDNV and AalDNV). However, the overall genomic organization and genome size of HPV were different from these parvoviruses, for instance, the non-overlapping of NS1 and NS2, the larger VP gene, and the bigger genome size. This suggested that this HPV virus is a new type in Parvoviridae family. We therefore propose to rename this virus P. monodon densovirus (PmDNV). © 2005 Elsevier Inc. All rights reserved.

Subject(s)

Amino acid
 Capsid protein
 Helicase
 Nucleotide
 Amino acid sequence
 Article
 Controlled study
 Genetic analysis
 Genetic organization
 Genome size
 Hepatopancreas
 Inverted terminal repeat
 Nonhuman
 Nucleotide sequence
 Open reading frame
 Parvovirus
 Phylogeny
 Priority journal
 Protein function
 Shrimp
 Thailand
 Virus isolation
 Amino Acid Sequence
 Animals
 Base Sequence
 Capsid Proteins
 Densovirinae
 Densovirus
 DNA Helicases
 DNA, Viral
 DNA-Binding Proteins
 Genome, Viral
 Molecular Weight
 Open Reading Frames
 Penaeidae
 Phylogeny
 Protein Processing, Post-Translational
 Protein Structure, Tertiary
 Sequence Analysis, DNA
 Sequence Homology, Amino Acid
 Synteny
 Terminal Repeat Sequences
 Trans-Activators
 Viral Nonstructural Proteins
 Decapoda (Crustacea)
 Densovirus
 DNA viruses
 Hepatopancreatic parvovirus of penaeid shrimp
 Miridae
 Parvoviridae
 Parvovirus
 Penaeus monodon
 Thailand virus

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URI

https://hdl.handle.net/20.500.14740/5872

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Scopus: Year 1983-2021

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