Publication:
Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

10313613

DOI

10.1071/RD09128

Other identifier(s)

2-s2.0-77951784898

Rights Holder(s)

Scopus

Bibliographic Citation

Reproduction, Fertility and Development. Vol 22, No.5 (2010), p.788-795

Suggested Citation

Boonkusol D., Dinnyes A., Faisaikarm T., Sangsuwan P., Pratipnatalang N., Sa-Ardrit M., Saikhun K., Svasti S., Vadolas J., Winichagoon P., Fucharoen S., Kitiyanant Y. Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models. Reproduction, Fertility and Development. Vol 22, No.5 (2010), p.788-795. doi:10.1071/RD09128 Retrieved from: https://hdl.handle.net/20.500.14740/7582

Title

Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models

Author(s)

Boonkusol D.
 Dinnyes A.
 Faisaikarm T.
 Sangsuwan P.
 Pratipnatalang N.
 Sa-Ardrit M.
 Saikhun K.
 Svasti S.
 Vadolas J.
 Winichagoon P.
 Fucharoen S.
 Kitiyanant Y.

Abstract

The purpose of the present study was to investigate the efficiency of embryo cryopreservation for four transgenic (TG) thalassaemic mouse strains, which is a key element of the ongoing gene banking efforts for these highvalue animals. Heterozygous TG embryos were produced by breeding four lines of TG males to wild-type (WT) females (C57BL/6J). Intact two-cell embryos were cryopreserved by vitrification in straws using 35% ethylene glycol. Survival rates of cryopreserved embryos ranged between 91.1% (102/112) and 93.6% (176/188) without significant differences between the lines. In contrast, the paternal line had a significant effect on the development of these embryos to the blastocyst stage, which ranged from 50.6% (92/182) to 77.5% (79/102). This effect was also noted following embryo transfers, with implantation rates varying from 17.3% (19/110) to 78.1% (35/45). The results demonstrate that the in vivo developmental potential is significantly influenced byTG line and reveal a specific line effect on cryosurvival. All bacterial artificial chromosome transgenic fetuses developed from vitrified-warmed embryos showed expression of the human β-globin transgene. In conclusion, the present study shows a strongTG line effect on developmental competence following cryopreservation and the vitrification method was successful to bank the human β-globin TG-expressing mouse strains. © 2010 CSIRO.

Subject(s)

Beta globin
 Ethylene glycol
 Animal experiment
 Animal tissue
 Article
 Bacterial artificial chromosome
 Blastocyst
 Breeding line
 Controlled study
 Cryopreservation
 Embryo
 Embryo cryopreservation
 Embryo development
 Embryo transfer
 Female
 Gene expression
 Heterozygote
 In vivo study
 Male
 Mouse
 Mouse embryo
 Nidation
 Nonhuman
 Survival rate
 Transgene
 Transgenics
 Vitrification
 Wild type
 Animals
 Beta-Globins
 Chromosomes, Artificial, Bacterial
 Cryopreservation
 Embryo Culture Techniques
 Embryo Transfer
 Embryo, Mammalian
 Female
 Gene Expression
 Gene Transfer Techniques
 Heterozygote
 Humans
 Male
 Mice
 Mice, Inbred C57BL
 Mice, Inbred ICR
 Mice, Knockout
 Mice, Transgenic
 Thalassemia
 Animalia
 Bacteria (microorganisms)

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URI

https://hdl.handle.net/20.500.14740/7582

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Scopus: Year 1983-2021

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