Publication:
Characterization of stem cells from human ovarian follicular fluid; a potential source of autologous stem cell for cell-based therapy

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

9147470

DOI

10.1007/s13577-020-00439-2

Other identifier(s)

2-s2.0-85100344144

Rights Holder(s)

Scopus

Bibliographic Citation

Human Cell. Vol 34, No.2 (2021), p.300-309

Suggested Citation

Rungsiwiwut R., Numchaisrika P., Thuwanut P., Pruksananonda K. Characterization of stem cells from human ovarian follicular fluid; a potential source of autologous stem cell for cell-based therapy. Human Cell. Vol 34, No.2 (2021), p.300-309. doi:10.1007/s13577-020-00439-2 Retrieved from: https://hdl.handle.net/20.500.14740/7867

Title

Characterization of stem cells from human ovarian follicular fluid; a potential source of autologous stem cell for cell-based therapy

Author(s)

Rungsiwiwut R.
 Numchaisrika P.
 Thuwanut P.
 Pruksananonda K.

Abstract

Human ovarian follicular fluid (HOFF) contains proteins, extracellular matrixes necessary for growth and maturation of oocytes as well as granulosa cells. Epithelial cells and stem cells can be isolated from HOFF. However, information regarding stem cells derived from HOFF is still lacking. The objectives of the present study were to isolate, characterize, and differentiate cells derived from HOFF. HOFF was collected during the routine aspiration of oocytes in an assisted fertilization program and subjected to cell isolation, characterization, and in vitro culture. After 24 h of culture, different cell morphologies including epithelial-like-, neural-like- and fibroblast-like cells were observed. Immunocytochemistry reveals the expression of pluripotent stem cell markers (OCT4, NANOG, SSEA4), epithelial marker (CK18), FSH- and LH-receptor. For in vitro culture, the isolated cells were continuously cultured in a growth medium; alpha MEM containing 10% FBS and epidermal growth factor (EGF). After 2 weeks of in vitro culture, cells with fibroblast-like morphology dominantly grow in the culture vessels and resemble mesenchymal stem cells (MSCs). HOFF-derived cells exhibited MSC expression of CD44, CD73, CD90, CD105, CD146, and STRO-1, and were capable of differentiation into osteoblasts, chondrocytes, and adipocytes. After induction of neural differentiation, HOFF-derived cells formed spheroidal structures and expressed neural stem cell markers including Nestin, β-tubulin III, and O4. Besides, the oocyte-like structure was observed after prolonged culture of HOFF. In conclusion, cells derived from follicular fluid exhibited stem cell characteristics, which could be useful for regenerative medicine applications and cell-based therapies. © 2021, Japan Human Cell Society.

Subject(s)

5' nucleotidase
 Beta tubulin
 CD146 antigen
 Cytokeratin 18
 Endoglin
 Epidermal growth factor
 Follitropin
 Hermes antigen
 Luteinizing hormone receptor
 Nestin
 O4 protein
 Octamer transcription factor 4
 Protein
 Stage specific embryo antigen 4
 Stro 1 protein
 Thy 1 membrane glycoprotein
 Transcription factor NANOG
 Unclassified drug
 Leukocyte antigen
 Membrane antigen
 Nestin
 STRO-1 antigen, human
 Tubulin
 Adipocyte
 Article
 Autologous stem cell transplantation
 Cell differentiation
 Cell isolation
 Cell structure
 Chondrocyte
 Controlled study
 Female
 Fibroblast
 Human
 Human cell
 Immunocytochemistry
 In vitro study
 Mesenchymal stem cell
 Oocyte
 Osteoblast
 Ovary follicle fluid
 Priority journal
 Protein expression
 Stem cell culture
 Biological therapy
 Cell culture
 Cell differentiation
 Cell separation
 Chemistry
 Cytology
 In vitro fertilization
 Metabolism
 Ovary follicle
 Ovary follicle fluid
 Physiology
 Pluripotent stem cell
 Procedures
 Regenerative medicine
 Antigens, CD
 Antigens, Surface
 Cell Differentiation
 Cell Separation
 Cell- and Tissue-Based Therapy
 Cells, Cultured
 Female
 Fertilization in Vitro
 Follicular Fluid
 Humans
 Nestin
 Ovarian Follicle
 Pluripotent Stem Cells
 Regenerative Medicine
 Tubulin

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URI

https://hdl.handle.net/20.500.14740/7867

Collections

Scopus: Year 1983-2021

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