Publication: Enhancement of TNF-α induced antiproliferation in human cervical cancer cells by extracts from Thai medicinal plants
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Issued Date
2014
Resource Type
File Type
application/pdf
ISSN
5677572
Other identifier(s)
2-s2.0-84899560993
Rights Holder(s)
Scopus
Bibliographic Citation
Acta Horticulturae. Vol 1023, (2014), p.193-199
Suggested Citation
Managit C., Sithisarn P., Jarikasem S., Sakurai H., Saiki I. Enhancement of TNF-α induced antiproliferation in human cervical cancer cells by extracts from Thai medicinal plants. Acta Horticulturae. Vol 1023, (2014), p.193-199. doi:10.17660/actahortic.2014.1023.28 Retrieved from: https://hdl.handle.net/20.500.14740/6969
Author(s)
Abstract
Human cervical cancer is known to be a dreadful disease with high resistance to chemotherapeutic drugs in clinical use. Natural products are valuable sources for development of anticancer agents. Many plant-derived anticancer compounds were reported to modulate signal transduction pathway associated with cell proliferation and apoptosis. One well-known pathway is via the enhancement of TNF-α, a cytokine that can induce apoptotic cell death and inhibit tumorigenesis. The purpose of this study is to evaluate TNF-α antiproliferative synergistic activity of Thai medicinal plant extracts. The antiproliferative effects were determined in human cervical cancer (HeLa) cells using WST-1 cell proliferating assay. Among twenty six different tested extracts at the concentration of 30 μg ml-1, 95% ethanolic extract from stems of Derris scandens, aqueous extract from leaves of Azadirachta indica, aqueous extract from leaves of A. indica var. siamensis and 95% ethanolic extract from roots of Amomum biflorum significantly decreased viability of HeLa cells treated with TNF-α (20 ng/ml). However, only 95% ethanolic extract from roots of A. biflorum combined with TNF-α significantly decreased viability of HeLa cells to 70.96±2.25% cell viability, when compared to cell viability of HeLa cells treated with only plant extract (93.84±10.73%). Data suggested that this plant extract enhanced TNF-α antiproliferative activity in HeLa cells and it has potential for further development as chemotherapeutic agent for anticancer therapy via the enhancement of TNF-α induced apoptosis of cancer cells. © ISHS.