Publication:
Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

10504648

DOI

10.1016/j.fsi.2018.10.015

Other identifier(s)

2-s2.0-85055025457

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Fish and Shellfish Immunology. Vol 84, (2019), p.441-450

Suggested Citation

Vaniksampanna A., Longyant S., Charoensapsri W., Sithigorngul P., Chaivisuthangkura P. Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii. Fish and Shellfish Immunology. Vol 84, (2019), p.441-450. doi:10.1016/j.fsi.2018.10.015 Retrieved from: https://hdl.handle.net/20.500.14740/5606

Title

Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii

Author(s)

Vaniksampanna A.
 Longyant S.
 Charoensapsri W.
 Sithigorngul P.
 Chaivisuthangkura P.

Abstract

Spätzle protein is an extracellular ligand of Toll receptor in Toll signaling pathway involved in the embryonic dorsoventral patterning and in the innate immunity. In this study, a spätzle gene of freshwater prawn, Macrobrachium rosenbergii (MrSpz) was isolated and characterized. The open reading frame of MrSpz consisted of 747 nucleotides encoding 248 amino acid residues containing a signal peptide and C-terminal spätzle activated domain. MrSpz shared high similarity to spätzle of Fenneropenaeus chinensis (FcSpz) at 92% identity and Marsupenaeus japonicus (MjSpz) at 83% identity. Phylogenetic analysis was performed and the results revealed that MrSpz was a member of the clade containing LvSpz3 of Litopenaeus vannamei, FcSpz and Penaeus monodon spätzle protein. The expression distribution at transcriptional level in various tissues of normal prawn revealed that the MrSpz was detected in gills, heart and hepatopancreas while no expression was observed in hemocyte, muscle and stomach. In the Aeromonas caviae challenged prawn, the expression level of MrSpz in hemocyte was increased gradually at 6, 12 and 24 h post-injection. Furthermore, in MrSpz knocked down prawn injected with Aeromonas caviae, the mortality rate were higher than that of non-related dsRNA group and control group. These results suggest that MrSpz protein may play a key role in the innate immunity of M. rosenbergii, especially in response to Gram-negative bacteria A. caviae invasion. © 2018 Elsevier Ltd

Subject(s)

Amino acid
 Complementary DNA
 Cysteine
 Nucleotide
 Protein
 Signal peptide
 Spatzle protein
 Unclassified drug
 Arthropod protein
 5' untranslated region
 Aeromonas caviae
 Allele
 Amino acid sequence
 Animal tissue
 Artemia
 Artemia sinica
 Article
 Bacterial count
 Bacterium colony
 Blood cell
 Bombyx mori
 Codon
 Consensus sequence
 Controlled study
 Culex quinquefasciatus
 Danaus plexippus
 Daphnia magna
 DNA sequence
 Drosophila melanogaster
 Fenneropenaeus chinensis
 Folsomia candida
 Gene knockdown
 Gill
 Heart
 Hepatopancreas
 Lepeophtheirus salmonis
 Litopenaeus vannamei
 Macrobrachium rosenbergii
 Marsupenaeus japonicus
 Mortality rate
 Nonhuman
 Nucleotide sequence
 Open reading frame
 Orchesella cincta
 Penaeus monodon
 Phylogenetic tree
 Plutella xylostella
 Priority journal
 Protein expression
 RNA interference
 Tribolium castaneum
 Animal
 Chemistry
 Gene expression profiling
 Gene expression regulation
 Genetics
 Immunology
 Innate immunity
 Palaemonidae
 Phylogeny
 Physiology
 Sequence alignment
 Aeromonas caviae
 Amino Acid Sequence
 Animals
 Arthropod Proteins
 Base Sequence
 Gene Expression Profiling
 Gene Expression Regulation
 Immunity, Innate
 Palaemonidae
 Phylogeny
 Sequence Alignment

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URI

https://hdl.handle.net/20.500.14740/5606

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Scopus: Year 1983-2021

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