Publication: Temperature dependency of bidirectional flux in the rat intestine subjected to graded ischemia.
0
0
Issued Date
2009
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-77957315060
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Journal of the Medical Association of Thailand = Chotmaihet thangphaet. Vol 92 Suppl 3, (2009), p.S15-23
Suggested Citation
Wattanasirichaigoon S. Temperature dependency of bidirectional flux in the rat intestine subjected to graded ischemia.. Journal of the Medical Association of Thailand = Chotmaihet thangphaet. Vol 92 Suppl 3, (2009), p.S15-23. Retrieved from: https://hdl.handle.net/20.500.14740/7698
Author(s)
Abstract
This study examined the effect of temperature and ischemia on permeation of fluorescently-labeled dextran (M.W. = 4 kDa; FD4) across rat intestinal mucosa. Permeability was evaluated ex vivo using an everted gut sac technique in both the mucosal-to-serosal (M-->S) and serosal-to-mucosal (S-->M) directions. At baseline (B), 30-min of ischemia (I-30) and 60-min of ischemia (I-60), intestinal segments were prepared and incubated at 37 degrees C, 15 degrees C and 4 degrees C for 30 min. Clearance (nl/min/cm2) was calculated based on the accumulated amount of FD4 at 30 min. Both M-->S and S-->M fluxes increased with increasing temperature at B, I-30 and I-60. Ischemic gut (I-30 and I-60) had about a three-fold higher (M-->S)/(S-->M) flux ratio than that of normal gut (p < 0.001). At 4 degrees C, neither M-->S nor S-->M flux was different between B and I-30, but both M-->S and S-->M fluxes significantly increased at I-60, suggesting an increase in permeation via a passive mechanism. Increased bidirectional fluxes at 37 degrees C were obtained in the I-30 and I-60 gut sacs when compared to B. We conclude that FD4 is actively transported across the intestinal mucosa in the S-->M direction and that ischemic injury increases passive diffusion of the probe across the gut wall.
Subject(s)
Fluorescent dye
Analysis of variance
Animal
Article
Body temperature
Capillary permeability
Cytology
Intestine
Intestine mucosa
Ischemia
Pathophysiology
Rat
Sprague Dawley rat
Vascularization
Analysis of Variance
Animals
Body Temperature
Capillary Permeability
Fluorescent Dyes
Intestinal Mucosa
Intestines
Ischemia
Rats
Rats, Sprague-Dawley
Analysis of variance
Animal
Article
Body temperature
Capillary permeability
Cytology
Intestine
Intestine mucosa
Ischemia
Pathophysiology
Rat
Sprague Dawley rat
Vascularization
Analysis of Variance
Animals
Body Temperature
Capillary Permeability
Fluorescent Dyes
Intestinal Mucosa
Intestines
Ischemia
Rats
Rats, Sprague-Dawley