Publication:
Targeted deletion of Atg5 reveals differential roles of autophagy in keratin K5-expressing epithelia

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

0006291X

DOI

10.1016/j.bbrc.2012.11.090

Other identifier(s)

2-s2.0-84872312203

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Biochemical and Biophysical Research Communications. Vol 430, No.2 (2013), p.689-694

Suggested Citation

Sukseree S., Rossiter H., Mildner M., Pammer J., Buchberger M., Gruber F., Watanapokasin R., Tschachler E., Eckhart L. Targeted deletion of Atg5 reveals differential roles of autophagy in keratin K5-expressing epithelia. Biochemical and Biophysical Research Communications. Vol 430, No.2 (2013), p.689-694. doi:10.1016/j.bbrc.2012.11.090 Retrieved from: https://hdl.handle.net/20.500.14740/6730

Title

Targeted deletion of Atg5 reveals differential roles of autophagy in keratin K5-expressing epithelia

Author(s)

Sukseree S.
 Rossiter H.
 Mildner M.
 Pammer J.
 Buchberger M.
 Gruber F.
 Watanapokasin R.
 Tschachler E.
 Eckhart L.

Abstract

Autophagy contributes to the homeostasis of many tissues, yet its role in epithelia is incompletely understood. A recent report proposed that Atg5-dependent autophagy in thymic epithelial cells is essential for their function in the negative selection of self-reactive T-cells and, thus, for the suppression of tissue inflammation. Here we crossed mice carrying floxed alleles of the Atg5 gene with mice expressing the Cre recombinase under the control of the keratin K5 promoter to suppress autophagy in all K5-positive epithelia. The efficiency of autophagy abrogation was confirmed by immunoanalyses of LC3, which was converted to the autophagy-associated LC3-II form in normal but not Atg5-deficient cells, and of p62, which accumulated in Atg5-deficient cells. Mice carrying the epithelium-specific deletion of Atg5 showed normal weight gain, absence of tissue inflammation, and a normal morphology of the thymic epithelium. By contrast, autophagy-deficient epithelial cells of the preputial gland showed aberrant eosinophilic staining in histology and premature degradation of nuclear DNA during terminal differentiation. Taken together, the results of this study suggest that autophagy is dispensable for the suppression of autoimmunity by thymic epithelial cells but essential for normal differentiation of the preputial gland in mice. © 2012 Elsevier Inc.

Subject(s)

Autophagy protein 5
 Cell nucleus DNA
 Cre recombinase
 Keratin
 Keratin k5
 Protein p62
 Unclassified drug
 Allele
 Animal experiment
 Animal tissue
 Article
 Autophagy
 Cell differentiation
 Cell structure
 Controlled study
 DNA degradation
 Epithelium cell
 Gene deletion
 Gene expression
 Histopathology
 Immunoassay
 Immunofluorescence test
 Inflammation
 Mouse
 Nick end labeling
 Nonhuman
 Priority journal
 Promoter region
 Reverse transcription polymerase chain reaction
 Weight gain
 Western blotting
 Animals
 Autoimmunity
 Autophagy
 Body Weight
 Cell Differentiation
 Epithelial Cells
 Gene Deletion
 Gene Targeting
 Keratin-5
 Mice
 Mice, Transgenic
 Microtubule-Associated Proteins
 Thymus Gland
 Weight Gain
 Mus

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URI

https://hdl.handle.net/20.500.14740/6730

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Scopus: Year 1983-2021

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