Publication:
High transfection efficiency of cationic lipids with asymmetric acyl-Cholesteryl hydrophobic tails

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Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

9476539

DOI

10.1002/chem.201001622

Other identifier(s)

2-s2.0-79952156409

Rights Holder(s)

Scopus

Bibliographic Citation

Chemistry - A European Journal. Vol 17, No.11 (2011), p.3287-3295

Suggested Citation

Radchatawedchakoon W., Krajarng A., Niyomtham N., Watanapokasin R., Yingyongnarongkul B.-E. High transfection efficiency of cationic lipids with asymmetric acyl-Cholesteryl hydrophobic tails. Chemistry - A European Journal. Vol 17, No.11 (2011), p.3287-3295. doi:10.1002/chem.201001622 Retrieved from: https://hdl.handle.net/20.500.14740/7365

Title

High transfection efficiency of cationic lipids with asymmetric acyl-Cholesteryl hydrophobic tails

Author(s)

Radchatawedchakoon W.
 Krajarng A.
 Niyomtham N.
 Watanapokasin R.
 Yingyongnarongkul B.-E.

Abstract

The ability of a nonviral gene delivery system to overcome extra- and intracellular barriers is a critical issue for the future clinical applications of gene therapy. In recent years much effort has been focused on the development of a variety of DNA carriers, and cationic liposomes have become the most common nonviral gene delivery system. One hundred and eighty novel cationic lipids with asymmetric acyl-cholesteryl hydrophobic tails were synthesized by parallel solid-phase chemistry. The liposomes were prepared and gel retardation assays were used to study the binding efficiency between the prepared liposome and the DNA. Transfection efficiencies of the lipids were evaluated against various mammalian cells, such as human embryonic kidney (HEK293), human cervical adenocarcinoma (HeLa), canine osteosarcoma (D17), colorectal adenocarcinoma (COLO 205), and human prostate adenocarcinoma (PC3) cells. The lipids with an acyl portion at the terminal part of the polyamine backbone exhibited higher transfection efficiency than those with the acyl portion as an internal part of the backbone. These compounds also showed higher transfection efficiency and lower cytotoxicity than the commercially available agents, Effectene, DOTAP, and DC-Chol. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Subject(s)

Cationic lipids
 DNA delivery
 Hydrophobic tails
 Nonviral vectors
 Solid-phase synthesis
 DNA
 Drug therapy
 Gene transfer
 Hydrophobicity
 Liposomes
 Mammals
 Nucleic acids
 Phospholipids
 Gene therapy
 1,2 dioleoyl glycero 3 phosphatidyl ethanolamine
 1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine
 Cation
 Cholesterol
 DNA
 Lipid
 Liposome
 Phosphatidylethanolamine
 Animal
 Article
 Cell strain HEK293
 Cell survival
 Chemical phenomena
 Chemistry
 Dog
 Drug effect
 Genetic transfection
 Human
 Metabolism
 Tumor cell line
 Animals
 Cations
 Cell Line, Tumor
 Cell Survival
 Cholesterol
 DNA
 Dogs
 HEK293 Cells
 Humans
 Hydrophobic and Hydrophilic Interactions
 Lipids
 Liposomes
 Phosphatidylethanolamines
 Transfection

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URI

https://hdl.handle.net/20.500.14740/7365

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Scopus: Year 1983-2021

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