Publication: Chrysin inhibits lymphangiogenesis in vitro
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0
Issued Date
2016
Resource Type
File Type
application/pdf
ISSN
9186158
Other identifier(s)
2-s2.0-84964692771
Rights Holder(s)
Scopus
Bibliographic Citation
Biological and Pharmaceutical Bulletin. Vol 39, No.4 (2016), p.466-472
Suggested Citation
Prangsaengtong O., Athikomkulchai S., Xu J., Koizumi K., Inujima A., Shibahara N., Shimada Y., Tadtong S., Awale S. Chrysin inhibits lymphangiogenesis in vitro. Biological and Pharmaceutical Bulletin. Vol 39, No.4 (2016), p.466-472. doi:10.1248/bpb.b15-00543 Retrieved from: https://hdl.handle.net/20.500.14740/5479
Abstract
The induction of lymphangiogenesis is an important process to promote cancer growth and cancer metastasis via the lymphatic system. Identifying the compounds that can prevent lymphangiogenesis for cancer therapy is urgently required. Chrysin, 5,7-dihydroxyflavone, a natural flavone extracted from Thai propolis, was used to investigate the effect on the lymphangiogenesis process of TR-LE, rat lymphatic endothelial cells. In this study, maximal nontoxic doses of chrysin on TR-LE cells were selected by performing a proliferation assay. The process of lymphangiogenesis in vitro was determined by cord formation assay, adhesion assay and migration assay. Chrysin at a nontoxic dose (25 μM) significantly inhibited cord formation, cell adhesion and migration of TR-LE cells when compared with the control group. We also found that chrysin significantly induced vascular endothelial growth factor C (VEGF-C) mRNA expression and nitric oxide (NO) production in TR-LE cells which was involved in decreasing the cord formation of TR-LE cells. In conclusion, we report for the first time that chrysin inhibited the process of lymphangiogenesis in an in vitro model. This finding may prove to be a natural compound for anti-lymphangiogenesis that could be developed for use in cancer therapy. © 2016 The Pharmaceutical Society of Japan.
Subject(s)
Chrysin
Messenger RNA
Nitric oxide
Propolis
Vasculotropin C
Chrysin
Flavonoid
Messenger RNA
Nitric oxide
Vasculotropin C
Animal cell
Antiproliferative activity
Apis mellifera
Article
Cancer therapy
Cell adhesion assay
Cell assay
Cell migration assay
Cell proliferation assay
Concentration response
Controlled study
Cord formation assay
Drug structure
Endothelium cell
Gene expression
In vitro study
Lymphangiogenesis
Lymphatic endothelial cell
Nonhuman
Rat
Real time polymerase chain reaction
Animal
Bee
Cell adhesion
Cell line
Cell motion
Cell proliferation
Drug effects
Genetics
Lymphangiogenesis
Metabolism
Animals
Bees
Cell Adhesion
Cell Line
Cell Movement
Cell Proliferation
Endothelial Cells
Flavonoids
Lymphangiogenesis
Nitric Oxide
Propolis
Rats
RNA, Messenger
Vascular Endothelial Growth Factor C
Messenger RNA
Nitric oxide
Propolis
Vasculotropin C
Chrysin
Flavonoid
Messenger RNA
Nitric oxide
Vasculotropin C
Animal cell
Antiproliferative activity
Apis mellifera
Article
Cancer therapy
Cell adhesion assay
Cell assay
Cell migration assay
Cell proliferation assay
Concentration response
Controlled study
Cord formation assay
Drug structure
Endothelium cell
Gene expression
In vitro study
Lymphangiogenesis
Lymphatic endothelial cell
Nonhuman
Rat
Real time polymerase chain reaction
Animal
Bee
Cell adhesion
Cell line
Cell motion
Cell proliferation
Drug effects
Genetics
Lymphangiogenesis
Metabolism
Animals
Bees
Cell Adhesion
Cell Line
Cell Movement
Cell Proliferation
Endothelial Cells
Flavonoids
Lymphangiogenesis
Nitric Oxide
Propolis
Rats
RNA, Messenger
Vascular Endothelial Growth Factor C