Publication:
Molecular identification of poisonous mushrooms using nuclear ITS region and peptide toxins: A retrospective study on fatal cases in Thailand

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Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

18803989

DOI

10.2131/jts.41.65

Other identifier(s)

2-s2.0-84954349887

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Toxicological Sciences. Vol 41, No.1 (2016), p.65-76

Suggested Citation

Parnmen S., Sikaphan S., Leudang S., Boonpratuang T., Rangsiruji A., Naksuwankul K. Molecular identification of poisonous mushrooms using nuclear ITS region and peptide toxins: A retrospective study on fatal cases in Thailand. Journal of Toxicological Sciences. Vol 41, No.1 (2016), p.65-76. doi:10.2131/jts.41.65 Retrieved from: https://hdl.handle.net/20.500.14740/5738

Title

Molecular identification of poisonous mushrooms using nuclear ITS region and peptide toxins: A retrospective study on fatal cases in Thailand

Author(s)

Parnmen S.
 Sikaphan S.
 Leudang S.
 Boonpratuang T.
 Rangsiruji A.
 Naksuwankul K.

Abstract

Cases of mushroom poisoning in Thailand have increased annually. During 2008 to 2014, the cases reported to the National Institute of Health included 57 deaths; at least 15 died after ingestion of amanitas, the most common lethal wild mushrooms inhabited. Hence, the aims of this study were to identify mushroom samples from nine clinically reported cases during the 7-year study period based on nuclear ITS sequence data and diagnose lethal peptide toxins using a reversed phase LC-MS method. Nucleotide similarity was identified using BLAST search of the NCBI database and the Barcode of Life Database (BOLD). Clade characterization was performed by maximum likelihood and Bayesian phylogenetic approaches. Based on BLAST and BOLD reference databases our results yielded high nucleotide similarities of poisonous mushroom samples to A. exitialis and A. fuliginea. Detailed phylogenetic analyses showed that all mushroom samples fall into their current classification. Detection of the peptide toxins revealed the presence of amatoxins and phallotoxins in A. exitialis and A. fuliginea. In addition, toxic α-amanitin was identified in a new provisional species, Amanita sp.1, with the highest toxin quantity. Molecular identification confirmed that the mushrooms ingested by the patients were members of the lethal amanitas in the sections Amanita and Phalloideae. In Thailand, the presence of A. exitialis was reported here for the first time and all three poisonous mushroom species provided new and informative data for clinical studies. © 2016, The Japanese Society of Toxicology. All rights reserved.

Subject(s)

Alpha amanitin
 Beta amanitin
 Phallacidin
 Phalloidin
 Amanitin
 Phallotoxin
 Acute kidney failure
 Acute liver failure
 Article
 DNA extraction
 DNA sequence
 Genetic variability
 Heart arrest
 Liquid chromatography
 Mass spectrometry
 Nonhuman
 Phylogeny
 Poisonous mushroom
 Polymerase chain reaction
 Protein analysis
 Protein expression
 Retrospective study
 Sequence alignment
 Sequence analysis
 Thailand
 Amanita
 Classification
 Genetic database
 Genetics
 Human
 Isolation and purification
 Mushroom poisoning
 Procedures
 Amanita
 Amanitins
 Chromatography, Liquid
 Databases, Genetic
 Humans
 Mass Spectrometry
 Mushroom Poisoning
 Retrospective Studies
 Sequence Analysis, DNA
 Thailand

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URI

https://hdl.handle.net/20.500.14740/5738

Collections

Scopus: Year 1983-2021

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