Publication: Development of miniaturized electrochemical devices for detection of dopamine, l-cysteine and anti-sars-cov-2 antibodies
1
0
Issued Date
2021-12-17
Resource Type
Language
eng
File Type
application/pdf
Access Rights
Open Access
Rights
ผลงานนี้เผยแพร่ภายใต้ สัญญาอนุญาตครีเอทีฟคอมมอนส์แบบ แสดงที่มา-ไม่ใช้เพื่อการค้า-ไม่ดัดแปลง 4.0 (CC BY-NC-ND 4.0)
Rights Holder(s)
Srinakharinwirot University
Suggested Citation
Wisarut Khamcharoen, วิศรุต ขำเจริญ (2021). Development of miniaturized electrochemical devices for detection of dopamine, l-cysteine and anti-sars-cov-2 antibodies. Retrieved from: https://hdl.handle.net/20.500.14740/54348
Alternative Title(s)
การพัฒนาอุปกรณ์ย่อส่วนทางเคมีไฟฟ้าสำหรับการตรวจวัดโดพามีน แอล-ซิสเทอีน และแอนติบอดีต้านเชื้อไวรัสซาร์ส-โควี-ทู
Author(s)
Advisor(s)
Organization
Abstract
This dissertation aims to develop novel electrochemical devices for the detection of important target analytes, which can be divided into two parts. The first part concerned the development of electrochemical paper-based analytical devices for clinical and food applications, and there were two subprojects in this section. The first subproject is the fabrication of electrochemical microfluidic paper-based analytical device (eµPAD) and developed for the highly selective and sensitive detection of dopamine (DA). Nafion was deposited into paper-based microchannel to increase selectivity and multilayered eµPAD was subsequently designed for the flow of the solution. The next subproject is a novelty of poly(cysteine) modified screen-printed graphene electrode, developed for the electroanalysis of L-cysteine (Cys). Furthermore, an all-in-one origami paper-based analytical device was proposed to combine all of the procedures into one device and providing a more convenient method for end-users. The second part was the development of an electrochemical device for COVID-19 diagnosis. In this part, the sequential delivery pump-free microfluidic coupled with screen-printed electrodes (SPE), which were developed for simple and rapid detection of IgG antibodies against SARS-CoV-2 nucleocapsid protein (anti-N antibody). The immunological assay was performed on the nitrocellulose membrane where the SPE was aligned above to perform the sensitive electrochemical detection of anti-N antibodies. As previously mentioned, all electrochemical devices produced from this work had a great analytical performance and a low limit of detection that can be applied to detect specific target analytes in various real samples, e.g., urine, whole blood, and food samples.
-
-
Description
DOCTOR OF PHILOSOPHY (Ph.D.)
ปรัชญาดุษฎีบัณฑิต (ปร.ด.)
ปรัชญาดุษฎีบัณฑิต (ปร.ด.)
Degree Grantor(s)
Srinakharinwirot University