Publication:
Inhibitory effects of terrein on lung cancer cell metastasis and angiogenesis

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

1021335X

DOI

10.3892/or.2021.8045

Other identifier(s)

2-s2.0-85104285041

Rights Holder(s)

Scopus

Bibliographic Citation

Oncology Reports. Vol 45, No.6 (2021)

Suggested Citation

Buachan P., Namsa-Aid M., Sung H.K., Peng C., Sweeney G., Tanechpongtamb W. Inhibitory effects of terrein on lung cancer cell metastasis and angiogenesis. Oncology Reports. Vol 45, No.6 (2021). doi:10.3892/or.2021.8045 Retrieved from: https://hdl.handle.net/20.500.14740/7579

Title

Inhibitory effects of terrein on lung cancer cell metastasis and angiogenesis

Author(s)

Buachan P.
 Namsa-Aid M.
 Sung H.K.
 Peng C.
 Sweeney G.
 Tanechpongtamb W.

Abstract

Cancer metastasis is the leading cause of mortality in cancer patients. Over 70% of lung cancer patients are diagnosed at advanced or metastatic stages, and this results in an increased incidence of mortality. Terrein is a secondary bioactive fungal metabolite isolated from Aspergillus terreus. Numerous studies have demonstrated that terrein has anticancer properties, but in the present study, the cellular mechanisms underlying the inhibition of lung cancer cell metastasis by terrein was investigated for the first time. Using MTT assays, the cytotoxic effects of terrein were first examined in human lung cancer cells (A549 cells) and then compared with its cytotoxic effects in three noncancer control cell lines (Vero kidney, L6 skeletal muscle and H9C2 cardiomyoblast cells). The results indicated that terrein significantly reduced the viability of all these cells but exhibited a different level of toxicity in each cell type; these results revealed a specific concentration range in which the effect of terrein was specific to A549 cells. This significant cytotoxic effect of terrein in A549 cells was verified using LDH assays. It was then demon- strated that terrein attenuated the proliferation of A549 cells using IncuCyte image analysis. Regarding its antimetastatic effects, terrein significantly inhibited A549 cell adhesion, migration and invasion. In addition, terrein suppressed the angiogenic processes of A549 cells, including vascular endo- thelial growth factor (VEGF) secretion, capillary-like tube formation and VEGF/VEGFR2 interaction. These phenomena were accompanied by reduced protein levels of integrins, FAK, and their downstream mediators (e.g., PI3K, AKT, mTORC1 and P70S6K). All these data indicated that terrein was able to inhibit all the major metastatic processes in human lung cancer cells, which is crucial for cancer treatment. © 2021 Spandidos Publications. All rights reserved.

Subject(s)

Mammalian target of rapamycin complex 1
 Phosphatidylinositol 3 kinase
 Protein kinase B
 S6 kinase
 Terrein
 Vasculotropin
 Vasculotropin receptor 2
 Cyclopentane derivative
 KDR protein, human
 Terrein
 Vasculotropin A
 Vasculotropin receptor 2
 VEGFA protein, human
 Antiangiogenic activity
 Antiproliferative activity
 Article
 Cell adhesion
 Cell invasion
 Cell migration
 Controlled study
 Drug cytotoxicity
 Drug mechanism
 Human
 Human cell
 Lung metastasis
 Metastasis inhibition
 Migration inhibition
 Priority journal
 A-549 cell line
 Animal
 Aspergillus
 Cell motion
 Chemistry
 Chlorocebus aethiops
 Drug effect
 Isolation and purification
 Lung tumor
 Metabolism
 Neovascularization (pathology)
 Non small cell lung cancer
 Pathology
 Vascularization
 Vero cell line
 A549 Cells
 Animals
 Aspergillus
 Carcinoma, Non-Small-Cell Lung
 Cell Adhesion
 Cell Movement
 Chlorocebus aethiops
 Cyclopentanes
 Humans
 Lung Neoplasms
 Neovascularization, Pathologic
 Vascular Endothelial Growth Factor A
 Vascular Endothelial Growth Factor Receptor-2
 Vero Cells

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URI

https://hdl.handle.net/20.500.14740/7579

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Scopus: Year 1983-2021

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