Publication: Plant defense-related gene expression analysis of canker-infected lime seedling
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Issued Date
2020
Resource Type
File Type
application/pdf
ISSN
17551307
Other identifier(s)
2-s2.0-85079741356
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
IOP Conference Series: Earth and Environmental Science. Vol 432, No.1 (2020)
Suggested Citation
Sudyoung N., Tokuyama S., Krajangsang S., Pringsulaka O., Yeesin P., Koto R., Sarawaneeyaruk S. Plant defense-related gene expression analysis of canker-infected lime seedling. IOP Conference Series: Earth and Environmental Science. Vol 432, No.1 (2020). doi:10.1088/1755-1315/432/1/012007 Retrieved from: https://hdl.handle.net/20.500.14740/4680
Abstract
A serious problem for Paan-lime production in Thailand is a citrus canker disease caused by the bacterium Xanthomonas citri subsp. citri (Xcc). The use of antagonist against pathogenic Xcc is increasingly becoming popular. In this study, we investigated the effect of pathogenic Xcc and a bacterial antagonist on defense-related gene expression of Paan-lime. The ability of Xcc and the antagonist Pseudomonas aeruginosa SWUC02 to induce defense-related gene expression, including PR-1, Pt14, LRR8, and LOX gene, was assessed. We assured that all four defense-related genes are present in Paan-lime by analyzing the similarity of nucleotide sequences in parts of the genes against other citrus species. Using reverse-transcription PCR (RT-PCR), we showed that the antagonist is able to induce the expression of LRR8 gene at 24 hours post-inoculation, while Xcc induces PR-1 and LOX gene expression at the same time. Our results suggest that these defense-related genes alter their expressions in response to canker disease infection. Thus, we could use this group of genes as a biomarker for screening canker-resistance Paan-lime tree. © 2020 IOP Publishing Ltd. All rights reserved.